| RRC ID |
71682
|
| 著者 |
Kojima S, Yamaguchi H, Morita K, Ueno Y.
|
| タイトル |
Inhibitory effect of sodium 5,6-benzylidene ascorbate (SBA) on the elevation of melanin biosynthesis induced by ultraviolet-A (UV-A) light in cultured B-16 melanoma cells.
|
| ジャーナル |
Biol Pharm Bull
|
| Abstract |
Sodium 5,6-benzylidene ascorbate (SBA) is a conjugate of ascorbic acid (Asc) with benzyaldehyde. It has been found that the antioxidant activity of SBA is more stable and has a longer lifetime in living cells and organs than Asc. In this study, we investigated the effect of SBA on the induction of melanin in cultured melanoma (B-16) cells irradiated by UV-A. Melanin content of B-16 cells was significantly increased by UV-A irradiation. The induction was abolished by mannitol and particularly by superoxide dismutase, suggesting the involvement of O2- in the biosynthesis of melanin in cultured melanoma cells. This was theorized by the fact that the induction was also observed in B-16 cells treated with superoxide anion radicals chemically generated in the hypoxanthine/xanthine oxidase-reaction system, instead of UV-A irradiation. The induction of melanin caused by UV-A irradiation was suppressed by SBA in a dose-dependent manner. To elucidate the mechanism of this suppressive effect, the scavenging activity against O2-, and the inhibitory effect of SBA on tyrosinase activity were examined. ESR spectrometric analysis showed that SBA strongly scavenged O2-, and the presence of SBA in the medium remarkably inhibited the tyrosinase activity in cultured B-16 melanoma cells. It can be concluded that SBA effectively inhibits the melanin biosynthesis in B-16 melanoma cells induced by reactive oxygen species (ROS) generated by UV-A irradiation via tyrosinase.
|
| 巻・号 |
18(8)
|
| ページ |
1076-80
|
| 公開日 |
1995-8-1
|
| DOI |
10.1248/bpb.18.1076
|
| PMID |
8535399
|
| MeSH |
Antioxidants / pharmacology*
Ascorbic Acid / analogs & derivatives*
Ascorbic Acid / pharmacology
Benzylidene Compounds / pharmacology*
Free Radical Scavengers
Melanins / antagonists & inhibitors*
Melanins / biosynthesis
Melanoma, Experimental / metabolism*
Melanoma, Experimental / pathology
Reactive Oxygen Species
Tumor Cells, Cultured
Ultraviolet Rays*
|
| IF |
1.863
|
| リソース情報 |
| ヒト・動物細胞 |
B16 melanoma 4A5(RCB0557) |
