論文 - 詳細
| RRC ID | 71685 |
|---|---|
| 著者 | Hirano Y, Okuno M, Hayashi T, Goto K, Nakajima A. |
| タイトル | Cell-attachment activities of surface immobilized oligopeptides RGD, RGDS, RGDV, RGDT, and YIGSR toward five cell lines. |
| ジャーナル | J Biomater Sci Polym Ed |
| Abstract |
Tetrapeptides, Arg-Gly-Asp-Ser (RGDS), Arg-Gly-Asp-Val (RGDV), and Arg-Gly-Asp-Thr (RGDT), respectively, appearing in the cell-attachment domains of fibronectin, vitronectin, and collagen, and pentapeptide Tyr-Ile-Gly-Ser-Arg (YIGSR) appearing in B1 chain of laminin, were synthesized by liquid-phase procedure. Bioactivities of RGD, RGDX (X = S, V and T), YIGSR, and YIGSR-NH2 as cell recognition determinants were investigated by cell-attachment test using these oligopeptides immobilized to ethylene-acrylic acid copolymer (PEA) film. The cell lines used were A431, NRK, CHO-K1, HeLa.S3, and RLC-16 cells. It was found that the residue X in RGDX plays an important role for cell-attachment activity of RGDX, and, regarding YIGSR, introduction of NH2 residue at the C-terminal of the pentapeptide enhances the cell-attachment activity. |
| 巻・号 | 4(3) |
| ページ | 235-43 |
| 公開日 | 1993-1-1 |
| DOI | 10.1163/156856293x00546 |
| PMID | 8476793 |
| MeSH | Acrylic Resins Amino Acid Sequence Animals Biocompatible Materials* Cell Adhesion / drug effects* Cell Line Cricetinae Humans Materials Testing Molecular Sequence Data Oligopeptides / chemistry Oligopeptides / pharmacology* Polyethylenes Rats Structure-Activity Relationship Surface Properties |
| IF | 2.69 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CHO-K1(RCB0285) NRK(RCB0043) A431(RCB0202) |