Reference - Detail
| RRC ID | 71705 |
|---|---|
| Author | Shiraishi M, Chuu YH, Sekiya T. |
| Title | Isolation of DNA fragments associated with methylated CpG islands in human adenocarcinomas of the lung using a methylated DNA binding column and denaturing gradient gel electrophoresis. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
We have constructed a library of DNA fragments heavily methylated in human adenocarcinomas of the lung to permit the comprehensive isolation of methylated CpG islands in cancer. Heavily methylated genomic DNA fragments from tumors of nine male patients were enriched using a methylated DNA binding column and used for construction of the library. From this library, DNA fragments having properties of CpG islands were isolated on the basis of their reduced rate of strand dissociation during denaturing gradient gel electrophoresis. Approximately 1,000 clones, corresponding to 0.3% of the library were analyzed, and nine DNA fragments were identified as being associated with CpG islands that were methylated in tumor DNA. One CpG island was methylated specifically in tumor DNA, whereas the remaining eight CpG islands were methylated both in normal and tumor DNA derived from the same patients. Our results suggest that the number of CpG islands methylated specifically in tumors is not large. The library, which contains DNA fragments from methylated CpG islands comprehensively, is expected to be valuable when elucidating epigenetic processes involved in carcinogenesis. |
| Volume | 96(6) |
| Pages | 2913-8 |
| Published | 1999-3-16 |
| DOI | 10.1073/pnas.96.6.2913 |
| PMID | 10077611 |
| PMC | PMC15869 |
| MeSH | Adenocarcinoma / genetics* Chromatography / methods* CpG Islands / genetics* DNA Methylation DNA, Neoplasm / genetics DNA, Neoplasm / isolation & purification* Electrophoresis / methods* Humans Lung Neoplasms / genetics* Male Molecular Sequence Data Polymerase Chain Reaction |
| IF | 9.412 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 48 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Human and Animal Cells | A549(RCB0098) LC-2/ad(RCB0440) |