Reference - Detail
| RRC ID | 71708 |
|---|---|
| Author | Hino M, Kurogi K, Okubo MA, Murata-Hori M, Hosoya H. |
| Title | Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
One of the monoclonal antibodies raised against mitotic HeLa cells (termed as mH3) recognized a 27-kDa protein and stained microtubules in the mitotic spindles of HeLa cells. Immunoscreening of a HeLa cDNA library revealed that mH3 antigen is a small heat shock protein, HSP27. Immunoprecipitation analysis using mH3 suggested that both alpha- and beta-tubulin are associated with HSP27. Further, sucrose-cushioned ultra centrifugation revealed that HSP27 is co-sedimented with taxol-stabilized microtubules. These results indicate that HSP27 associates with tubulin/microtubules in HeLa cells. |
| Volume | 271(1) |
| Pages | 164-9 |
| Published | 2000-4-29 |
| DOI | 10.1006/bbrc.2000.2553 |
| PII | S0006-291X(00)92553-1 |
| PMID | 10777697 |
| MeSH | Amino Acid Sequence Antibodies, Monoclonal / metabolism Fluorescent Antibody Technique, Indirect HeLa Cells Heat-Shock Proteins / metabolism* Humans Microscopy, Confocal Microtubules / metabolism* Mitosis Molecular Sequence Data Precipitin Tests Protein Binding Sequence Homology, Amino Acid Tubulin / metabolism* |
| IF | 2.985 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Wikipedia |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HeLa(RCB0007) |