| RRC ID |
71708
|
| Author |
Hino M, Kurogi K, Okubo MA, Murata-Hori M, Hosoya H.
|
| Title |
Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
One of the monoclonal antibodies raised against mitotic HeLa cells (termed as mH3) recognized a 27-kDa protein and stained microtubules in the mitotic spindles of HeLa cells. Immunoscreening of a HeLa cDNA library revealed that mH3 antigen is a small heat shock protein, HSP27. Immunoprecipitation analysis using mH3 suggested that both alpha- and beta-tubulin are associated with HSP27. Further, sucrose-cushioned ultra centrifugation revealed that HSP27 is co-sedimented with taxol-stabilized microtubules. These results indicate that HSP27 associates with tubulin/microtubules in HeLa cells.
|
| Volume |
271(1)
|
| Pages |
164-9
|
| Published |
2000-4-29
|
| DOI |
10.1006/bbrc.2000.2553
|
| PII |
S0006-291X(00)92553-1
|
| PMID |
10777697
|
| MeSH |
Amino Acid Sequence
Antibodies, Monoclonal / metabolism
Fluorescent Antibody Technique, Indirect
HeLa Cells
Heat-Shock Proteins / metabolism*
Humans
Microscopy, Confocal
Microtubules / metabolism*
Mitosis
Molecular Sequence Data
Precipitin Tests
Protein Binding
Sequence Homology, Amino Acid
Tubulin / metabolism*
|
| IF |
2.985
|
| Resource |
| Human and Animal Cells |
HeLa(RCB0007) |
