RRC ID 7171
Author Sasakura Y, Suzuki MM, Hozumi A, Inaba K, Satoh N.
Title Maternal factor-mediated epigenetic gene silencing in the ascidian Ciona intestinalis.
Journal Mol. Genet. Genomics
Abstract Epigenetic regulation of genes plays a critical role in achieving proper gene expression during development, and it has been reported that epigenetic modifications are associated with transposon silencing in many organisms. Here, we report a type of epigenetic gene silencing, maternal gfp/gene silencing (MGS), in the basal chordate Ciona intestinalis. A transgenic line of Ciona, Tg[MiTFr3dTPOG]45 (abbreviated as Tg45), which was created with the Minos transposon, has a tandemly arrayed insertion of gfp in the promoter region of Ci-CesA. Progeny of Tg45 showed a reduced level of GFP expression when eggs of Tg45 were fertilized with sperm of other gfp transgenic lines. Although the genotype is the same, animals developed from Tg45 sperm and the eggs of other transgenic lines did not exhibit this phenomenon, suggesting the involvement of a maternal cytoplasmic factor that influences GFP expression. The silencing starts during oogenesis and continues after fertilization without any tissue specificity. We found that post-transcriptional degradation of the gfp mRNA is involved in MGS.
Volume 283(1)
Pages 99-110
Published 2010-1
DOI 10.1007/s00438-009-0500-4
PMID 19946786
MeSH Animals Animals, Genetically Modified / genetics Animals, Genetically Modified / metabolism Ciona intestinalis / genetics* Ciona intestinalis / physiology DNA Methylation Female Gene Silencing* Green Fluorescent Proteins / genetics Male Oogenesis / genetics* RNA Stability
IF 2.734
Times Cited 15
WOS Category GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
C.intestinalis / (O.japonicus) Tg[MiTFr3dTPOG]45 Tg[MiTFr3dTPOG]84? Tg[MiTFr3dTPOG]117 Tg[MiTFr3dTPOG]153? Tg[MiCiEpiIG]3 Tg[MiCiEpiIG]4 Tg[MiCiCesAG]1 Tg[MiCiTnIG]1? Tg[MiCiTnIG]2 Tg[MiCiBraG]1 Tg[MiCiBraG]2 Tg[MiCiNutG]3 Tg[MiCiNutG]4 Tg[MiCiEf1aG]1 Tg[MiCiCesACsCesA-CiEpiIG]1? Tg[MiCiCesACsCesA-CiEpiIG]4? Tg[ISMiTSAdTPOG]1? Tg[MiCiTrlG]1 Tg[MiCiTnIGCiprmG]2 pMiDestF pMiEV pMiCiBraG pMiCiNutG pMiCiEf1aFG pSPCiEpiIG pMiCiEpiIG pMiCiCesAG pMiCiCesA(PstI)G pMiCiCesACsCesA pMiCiCesACsCesA-CiEpiIG pSPCiTrlG pMiCiTrlG pHTBeGFP