| RRC ID |
71711
|
| 著者 |
Lee DW, Akao T, Yamashita S, Katayama H, Maeda M, Saitoh H, Mizuki E, Ohba M.
|
| タイトル |
Noninsecticidal parasporal proteins of a Bacillus thuringiensis serovar shandongiensis isolate exhibit a preferential cytotoxicity against human leukemic T cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
A Bacillus thuringiensis isolate, 89-T-34-22, belonging to the serovar shandongiensis (H22) produced noninsecticidal and nonhemolytic proteins crystallizing into irregular-shaped parasporal inclusions. The proteins showed in vitro cytotoxicity to human cells, including cancer cells, only when activated by protease treatment. The human leukemic T (MOLT-4) cells were > 100 times more susceptible than HeLa and normal T cells to the proteins of 89-T-34-22. The cytotoxicity was dose dependent and the median effective concentration for the MOLT-4 was 3.5 microg/ml. The cytopathy induced by the 89-T-34-22 proteins was characterized by remarkable condensation of the nucleus and cell-ballooning. Five major parasporal proteins of 89-T-34-22, with molecular masses in the range of 16-160 kDa, shared no similarity with the previously reported proteins in terms of the N-terminal sequence.
|
| 巻・号 |
272(1)
|
| ページ |
218-23
|
| 公開日 |
2000-5-27
|
| DOI |
10.1006/bbrc.2000.2765
|
| PII |
S0006-291X(00)92765-7
|
| PMID |
10872830
|
| MeSH |
Amino Acid Sequence
Animals
Antineoplastic Agents / administration & dosage
Antineoplastic Agents / isolation & purification
Antineoplastic Agents / pharmacology*
Bacillus thuringiensis / chemistry*
Bacillus thuringiensis / genetics
Bacillus thuringiensis / ultrastructure
Bacterial Proteins / genetics
Bacterial Proteins / isolation & purification
Bacterial Proteins / pharmacology*
Cell Survival / drug effects
Dose-Response Relationship, Drug
HeLa Cells
Humans
Inclusion Bodies / chemistry
Leukemia, T-Cell / drug therapy*
Microscopy, Electron
Molecular Sequence Data
Molecular Weight
Spores, Bacterial / chemistry
Tumor Cells, Cultured
|
| IF |
2.985
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007)
MOLT-4(RCB0206) |
