RRC ID 71711
Author Lee DW, Akao T, Yamashita S, Katayama H, Maeda M, Saitoh H, Mizuki E, Ohba M.
Title Noninsecticidal parasporal proteins of a Bacillus thuringiensis serovar shandongiensis isolate exhibit a preferential cytotoxicity against human leukemic T cells.
Journal Biochem Biophys Res Commun
Abstract A Bacillus thuringiensis isolate, 89-T-34-22, belonging to the serovar shandongiensis (H22) produced noninsecticidal and nonhemolytic proteins crystallizing into irregular-shaped parasporal inclusions. The proteins showed in vitro cytotoxicity to human cells, including cancer cells, only when activated by protease treatment. The human leukemic T (MOLT-4) cells were > 100 times more susceptible than HeLa and normal T cells to the proteins of 89-T-34-22. The cytotoxicity was dose dependent and the median effective concentration for the MOLT-4 was 3.5 microg/ml. The cytopathy induced by the 89-T-34-22 proteins was characterized by remarkable condensation of the nucleus and cell-ballooning. Five major parasporal proteins of 89-T-34-22, with molecular masses in the range of 16-160 kDa, shared no similarity with the previously reported proteins in terms of the N-terminal sequence.
Volume 272(1)
Pages 218-23
Published 2000-5-27
DOI 10.1006/bbrc.2000.2765
PII S0006-291X(00)92765-7
PMID 10872830
MeSH Amino Acid Sequence Animals Antineoplastic Agents / administration & dosage Antineoplastic Agents / isolation & purification Antineoplastic Agents / pharmacology* Bacillus thuringiensis / chemistry* Bacillus thuringiensis / genetics Bacillus thuringiensis / ultrastructure Bacterial Proteins / genetics Bacterial Proteins / isolation & purification Bacterial Proteins / pharmacology* Cell Survival / drug effects Dose-Response Relationship, Drug HeLa Cells Humans Inclusion Bodies / chemistry Leukemia, T-Cell / drug therapy* Microscopy, Electron Molecular Sequence Data Molecular Weight Spores, Bacterial / chemistry Tumor Cells, Cultured
IF 2.985
Human and Animal Cells HeLa(RCB0007) MOLT-4(RCB0206)