論文 - 詳細
| RRC ID | 71711 |
|---|---|
| 著者 | Lee DW, Akao T, Yamashita S, Katayama H, Maeda M, Saitoh H, Mizuki E, Ohba M. |
| タイトル | Noninsecticidal parasporal proteins of a Bacillus thuringiensis serovar shandongiensis isolate exhibit a preferential cytotoxicity against human leukemic T cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
A Bacillus thuringiensis isolate, 89-T-34-22, belonging to the serovar shandongiensis (H22) produced noninsecticidal and nonhemolytic proteins crystallizing into irregular-shaped parasporal inclusions. The proteins showed in vitro cytotoxicity to human cells, including cancer cells, only when activated by protease treatment. The human leukemic T (MOLT-4) cells were > 100 times more susceptible than HeLa and normal T cells to the proteins of 89-T-34-22. The cytotoxicity was dose dependent and the median effective concentration for the MOLT-4 was 3.5 microg/ml. The cytopathy induced by the 89-T-34-22 proteins was characterized by remarkable condensation of the nucleus and cell-ballooning. Five major parasporal proteins of 89-T-34-22, with molecular masses in the range of 16-160 kDa, shared no similarity with the previously reported proteins in terms of the N-terminal sequence. |
| 巻・号 | 272(1) |
| ページ | 218-23 |
| 公開日 | 2000-5-27 |
| DOI | 10.1006/bbrc.2000.2765 |
| PII | S0006-291X(00)92765-7 |
| PMID | 10872830 |
| MeSH | Amino Acid Sequence Animals Antineoplastic Agents / administration & dosage Antineoplastic Agents / isolation & purification Antineoplastic Agents / pharmacology* Bacillus thuringiensis / chemistry* Bacillus thuringiensis / genetics Bacillus thuringiensis / ultrastructure Bacterial Proteins / genetics Bacterial Proteins / isolation & purification Bacterial Proteins / pharmacology* Cell Survival / drug effects Dose-Response Relationship, Drug HeLa Cells Humans Inclusion Bodies / chemistry Leukemia, T-Cell / drug therapy* Microscopy, Electron Molecular Sequence Data Molecular Weight Spores, Bacterial / chemistry Tumor Cells, Cultured |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) MOLT-4(RCB0206) |