Reference - Detail
| RRC ID | 71715 |
|---|---|
| Author | Akiba S, Hatazawa R, Ono K, Hayama M, Matsui H, Sato T. |
| Title | Transforming growth factor-alpha stimulates prostaglandin generation through cytosolic phospholipase A(2) under the control of p11 in rat gastric epithelial cells. |
| Journal | Br J Pharmacol |
| Abstract |
The regulatory effects of transforming growth factor (TGF)-alpha on phospholipase A(2) (PLA(2)) isozymes contributing to prostaglandin generation in rat gastric epithelial RGM1 cells were examined. Stimulation with TGF-alpha for 24 h time-dependently induced prostaglandin E(2) generation with an increase in cyclo-oxygenase-2 protein. The TGF-alpha-induced prostaglandin E(2) generation was suppressed by NS-398, a cyclo-oxygenase-2 inhibitor. TGF-alpha stimulated the activity and the protein synthesis of cytosolic PLA(2) (cPLA(2)). A time-dependent increase in cPLA(2) protein occurred in parallel with PGE(2) generation, which was inhibited by methyl arachidonyl fluorophosphonate (MAFP), a cPLA(2) inhibitor. However, no change in activity of secretory PLA(2) or Ca(+2)-independent PLA(2) was observed in the TGF-alpha-stimulated cells. Stimulation with the Ca(2+) ionophore A23187 for 10 min induced MAFP-sensitive arachidonic acid liberation. Interestingly, preincubation with TGF-alpha for 24 h diminished A23187-stimulated arachidonic acid liberation despite the increase in cPLA(2) protein. Under the conditions, TGF-alpha was found to increase p11, an endogenous cPLA(2) suppressor, also known as annexin II light chain. The TGF-alpha-induced increase in p11 was suppressed by tyrphostin AG1478, an inhibitor of tyrosine kinase of epidermal growth factor receptor, which was also found to restore the inhibition by TGF-alpha of A23187-stimulated arachidonic acid liberation. However, TGF-alpha did not alter protein levels of annexin II heavy chain. These results suggest that TGF-alpha stimulates prostaglandin generation through an increase in cPLA(2), the hydrolytic action of which may be under the control of p11. |
| Volume | 131(5) |
| Pages | 1004-10 |
| Published | 2000-11-1 |
| DOI | 10.1038/sj.bjp.0703637 |
| PII | 0703637 |
| PMID | 11053223 |
| PMC | PMC1572404 |
| MeSH | Animals Annexin A2* Arachidonic Acid / metabolism Calcimycin / pharmacology Calcium / metabolism Cell Line Cytosol / enzymology* Gastric Mucosa / cytology Gastric Mucosa / metabolism* Peptides / physiology* Phospholipases A / physiology* Prostaglandins / biosynthesis* Rats S100 Proteins* Transforming Growth Factor alpha / pharmacology* |
| IF | 7.73 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 9 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RGM1(RCB0876) |