RRC ID 71726
Author Hemagirri M, Sasidharan S.
Title In vitro antiaging activity of polyphenol rich Polyalthia longifolia (Annonaceae) leaf extract in Saccharomyces cerevisiae BY611 yeast cells.
Journal J Ethnopharmacol
Abstract ETHNOPHARMACOLOGICAL RELEVANCE:Polyalthia longifolia var. angustifolia Thw. (Annonaceae) is commonly used in traditional medicine as a tonic for rejuvenation and exhibiting good antioxidant activities.
AIM OF THE STUDY:To evaluate P. longifolia methanolic leaf extract (PLME) antiaging activity at 1 mg/mL in Saccharomyces cerevisiae BY611 yeast.
MATERIALS AND METHODS:The antiaging effect of PLME was studied via replicative lifespan assay, antioxidative stress assays, reactive oxygen species (ROS) determination, reduced glutathione (GSH) determination, superoxide dismutase (SOD) and Sirtuin 1 (SIRT1) genes regulation studies and SOD and SIRT1 proteins activities.
RESULTS:The PLME treatment increased the growth and prolonged the lifespan of the yeast significantly (p < 0.05) compared to the untreated yeast group. Besides, the PLME also protected the yeast from oxidative stress induced by 4-mM-H2O2 via decreasing (p < 0.05) the ROS from 143.207 to 127.223. The antioxidative action of PLME was proved by spot assay. Phloxine B staining was further confirmed the PLME antioxidative action of PLME, where more whitish-pink live yeast cells were observed. In addition, the PLME also enhanced GSH content significantly (p < 0.05) in yeast treated with PLME from 16.81 to 25.31 μmol. Furthermore, PLME increased the SOD and SIRT1 genes expression significantly (p < 0.05) with ΔCt values of 1.11 and 1.15, respectively. The significantly (p < 0.05) elevated SOD and SIRT1 protein activities were recorded as 51.54 U/mg Prot and 1716 ng/mL, respectively.
CONCLUSIONS:PLME exhibited good antiaging activities in S. cerevisiae, by modulating oxidative stress, enhancing GSH content, and increasing SOD and SIRT1 genes expression.
Volume 290
Pages 115110
Published 2022-5-23
DOI 10.1016/j.jep.2022.115110
PII S0378-8741(22)00148-9
PMID 35181488
MeSH Aging / drug effects* Antioxidants / pharmacology* Oxidative Stress / drug effects Plant Extracts / pharmacology* Polyalthia / chemistry* Polyphenols / pharmacology* Saccharomyces cerevisiae / drug effects* Sirtuin 1 / drug effects Superoxide Dismutase / drug effects
IF 3.69
Yeast BY611