論文 - 詳細
| RRC ID | 71726 |
|---|---|
| 著者 | Hemagirri M, Sasidharan S. |
| タイトル | In vitro antiaging activity of polyphenol rich Polyalthia longifolia (Annonaceae) leaf extract in Saccharomyces cerevisiae BY611 yeast cells. |
| ジャーナル | J Ethnopharmacol |
| Abstract |
ETHNOPHARMACOLOGICAL RELEVANCE:Polyalthia longifolia var. angustifolia Thw. (Annonaceae) is commonly used in traditional medicine as a tonic for rejuvenation and exhibiting good antioxidant activities. AIM OF THE STUDY:To evaluate P. longifolia methanolic leaf extract (PLME) antiaging activity at 1 mg/mL in Saccharomyces cerevisiae BY611 yeast. MATERIALS AND METHODS:The antiaging effect of PLME was studied via replicative lifespan assay, antioxidative stress assays, reactive oxygen species (ROS) determination, reduced glutathione (GSH) determination, superoxide dismutase (SOD) and Sirtuin 1 (SIRT1) genes regulation studies and SOD and SIRT1 proteins activities. RESULTS:The PLME treatment increased the growth and prolonged the lifespan of the yeast significantly (p < 0.05) compared to the untreated yeast group. Besides, the PLME also protected the yeast from oxidative stress induced by 4-mM-H2O2 via decreasing (p < 0.05) the ROS from 143.207 to 127.223. The antioxidative action of PLME was proved by spot assay. Phloxine B staining was further confirmed the PLME antioxidative action of PLME, where more whitish-pink live yeast cells were observed. In addition, the PLME also enhanced GSH content significantly (p < 0.05) in yeast treated with PLME from 16.81 to 25.31 μmol. Furthermore, PLME increased the SOD and SIRT1 genes expression significantly (p < 0.05) with ΔCt values of 1.11 and 1.15, respectively. The significantly (p < 0.05) elevated SOD and SIRT1 protein activities were recorded as 51.54 U/mg Prot and 1716 ng/mL, respectively. CONCLUSIONS:PLME exhibited good antiaging activities in S. cerevisiae, by modulating oxidative stress, enhancing GSH content, and increasing SOD and SIRT1 genes expression. |
| 巻・号 | 290 |
| ページ | 115110 |
| 公開日 | 2022-5-23 |
| DOI | 10.1016/j.jep.2022.115110 |
| PII | S0378-8741(22)00148-9 |
| PMID | 35181488 |
| MeSH | Aging / drug effects* Antioxidants / pharmacology* Oxidative Stress / drug effects Plant Extracts / pharmacology* Polyalthia / chemistry* Polyphenols / pharmacology* Saccharomyces cerevisiae / drug effects* Sirtuin 1 / drug effects Superoxide Dismutase / drug effects |
| IF | 3.69 |
| リソース情報 | |
| 酵母 | BY611 |