論文 - 詳細
| RRC ID | 71732 |
|---|---|
| 著者 | Buntru M, Gärtner S, Staib L, Kreuzaler F, Schlaich N. |
| タイトル | Delivery of multiple transgenes to plant cells by an improved version of MultiRound Gateway technology. |
| ジャーナル | Transgenic Res |
| Abstract |
At present, only few methods for the effective assembly of multigene constructs have been described. Here we present an improved version of the MultiRound Gateway technology, which facilitates plant multigene transformation. The system consists of two attL-flanked entry vectors, which contain an attR cassette, and a transformation-competent artificial chromosome based destination vector. By alternate use of the two entry vectors, multiple transgenes can be delivered sequentially into the Gateway-compatible destination vector. Multigene constructs that carried up to seven transgenes corresponding to more than 26 kb were assembled by seven rounds of LR recombination. The constructs were successfully transformed into tobacco plants and were stably inherited for at least two generations. Thus, our system represents a powerful, highly efficient tool for multigene plant transformation and may facilitate genetic engineering of agronomic traits or the assembly of genetic pathways for the production of biofuels, industrial or pharmaceutical compounds in plants. |
| 巻・号 | 22(1) |
| ページ | 153-67 |
| 公開日 | 2013-2-1 |
| DOI | 10.1007/s11248-012-9640-0 |
| PMID | 22972476 |
| MeSH | Arabidopsis / cytology Arabidopsis / genetics Chromosomes, Artificial Cloning, Molecular Gene Transfer Techniques* Genetic Engineering Genetic Vectors* Plants, Genetically Modified / genetics* Tobacco / cytology Tobacco / genetics Transgenes* |
| IF | 1.856 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpd00105 |