Reference - Detail
| RRC ID | 71744 |
|---|---|
| Author | Nakajima KP, Valansi C, Kurihara D, Sasaki N, Podbilewicz B, Higashiyama T. |
| Title | Live imaging-based assay for visualising species-specific interactions in gamete adhesion molecules. |
| Journal | Sci Rep |
| Abstract |
Successful gamete fusion requires species-specific membrane adhesion. However, the interaction of adhesion molecules in gametes is difficult to study in real time through low-throughput microscopic observation. Therefore, we developed a live imaging-based adhesion molecule (LIAM) assay to study gamete adhesion molecule interactions in cultured cells. First, we modified a fusion assay previously established for fusogens introduced into cultured cells, and confirmed that our live imaging technique could visualise cell-cell fusion in the modified fusion assay. Next, instead of fusogen, we introduced adhesion molecules including a mammalian gamete adhesion molecule pair, IZUMO1 and JUNO, and detected their temporal accumulation at the contact interfaces of adjacent cells. Accumulated IZUMO1 or JUNO was partly translocated to the opposite cells as discrete spots; the mutation in amino acids required for their interaction impaired accumulation and translocation. By using the LIAM assay, we investigated the species specificity of IZUMO1 and JUNO of mouse, human, hamster, and pig in all combinations. IZUMO1 and JUNO accumulation and translocation were observed in conspecific, and some interspecific, combinations, suggesting potentially interchangeable combinations of IZUMO1 and JUNO from different species. |
| Volume | 12(1) |
| Pages | 9609 |
| Published | 2022-6-10 |
| DOI | 10.1038/s41598-022-13547-w |
| PII | 10.1038/s41598-022-13547-w |
| PMID | 35688940 |
| PMC | PMC9187738 |
| MeSH | Animals Cell Adhesion Molecules / metabolism Cricetinae Fertilization / genetics Germ Cells / metabolism Immunoglobulins / metabolism Male Mammals / metabolism Membrane Proteins / metabolism Mice Receptors, Cell Surface* / metabolism Species Specificity Sperm-Ovum Interactions / genetics Spermatozoa* / metabolism Swine |
| IF | 3.998 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | BHK-21(RCB1423) |