RRC ID 71780
Author Todorova R.
Title Estimation of methods of protein delivery into mammalian cells--a comparative study by electroporation and bioporter assay.
Journal Prikl Biokhim Mikrobiol
Abstract To test the efficiency of some alternative methods for protein delivery into live cells were used rEGFP (recombinant Enhanced Green Fluorescent Protein) and HeLa cells as model system. The compared methods, electroporation (up to 10%) and Bioporter (up to 20%), deliver proteins and peptides to the cells with efficiency, enough for functional studies of mammalian cells. Thus, the delivery by either of both methods, followed by fluorescence activated cell sorting (FACS) of the cell population, can give a sufficient number of uniformly loaded cells for different studies including transcription assays and gene therapy.
Volume 45(4)
Pages 493-6
Published 2009-1-1
PMID 19764622
MeSH Animals CHO Cells COS Cells Chlorocebus aethiops Cricetinae Cricetulus Drug Delivery Systems / methods* Electroporation / methods* Genetic Therapy / methods Green Fluorescent Proteins / pharmacology* Humans Recombinant Proteins / pharmacology*
Human and Animal Cells PC-12(RCB0009)