RRC ID 71786
Author Kwiatkowska A, Zebrowski J, Oklejewicz B, Czarnik J, Halibart-Puzio J, Wnuk M.
Title The age-dependent epigenetic and physiological changes in an Arabidopsis T87 cell suspension culture during long-term cultivation.
Journal Biochem Biophys Res Commun
Abstract Plant cell suspension cultures represent good model systems applicable for both basic research and biotechnological purposes. Nevertheless, it is widely known that a prolonged in vitro cultivation of plant cells is associated with genetic and epigenetic instabilities, which may limit the usefulness of plant lines. In this study, the age-dependent epigenetic and physiological changes in an asynchronous Arabidopsis T87 cell culture were examined. A prolonged cultivation period was found to be correlated with a decrease in the proliferation rate and a simultaneous increase in the expression of senescence-associated genes, indicating that the aging process started at the late growth phase of the culture. In addition, increases in the heterochromatin-specific epigenetic markers, i.e., global DNA methylation, H3K9 dimethylation, and H3K27 trimethylation, were observed, suggesting the onset of chromatin condensation, a hallmark of the early stages of plant senescence. Although the number of live cells decreased with an increase in the age of the culture, the remaining viable cells retained a high potential to efficiently perform photosynthesis and did not exhibit any symptoms of photosystem II damage.
Volume 447(2)
Pages 285-91
Published 2014-5-2
DOI 10.1016/j.bbrc.2014.03.141
PII S0006-291X(14)00601-9
PMID 24709077
MeSH Arabidopsis / cytology* Arabidopsis / genetics Arabidopsis / physiology Cell Culture Techniques Cell Line Cell Proliferation Cell Survival Cellular Senescence / genetics* Cellular Senescence / physiology* Chlorophyll / analysis Chlorophyll A DNA Methylation Epigenesis, Genetic* Fluorescence Gene Expression Heterochromatin / chemistry Heterochromatin / metabolism Suspensions Time Factors
IF 2.985
Arabidopsis / Cultured plant cells, genes rpc00008