RRC ID |
7182
|
著者 |
Koeduka T, Shitan N, Kumano T, Sasaki K, Sugiyama A, Linley P, Kawasaki T, Ezura H, Kuzuyama T, Yazaki K.
|
タイトル |
Production of prenylated flavonoids in tomato fruits expressing a prenyltransferase gene from Streptomyces coelicolor A3(2).
|
ジャーナル |
Plant Biol (Stuttg)
|
Abstract |
Flavonoids are natural compounds found in many plants, including the important fruit crop, tomato. Prenylated flavonoids consist of a large group of compounds, which often exhibit antitumour, antibacterial and/or anti-androgen activities. In this study, we engineered the biosynthesis of prenylated flavonoids using a Streptomyces prenyltransferase HypSc (SCO7190) possessing broad-range substrate specificity, in tomato as a host plant. LC/MS/MS analysis demonstrated the generation of 3'-dimethylallyl naringenin in tomato fruits when recombinant HypSc protein was targeted to the plastids, whereas the recombinant protein hardly produced this compound in vitro. This is the first report confirming the accumulation of a prenylated flavonoid using a bacterial prenyltransferase in transgenic plants, and our results suggest that the product specificities of prenyltransferases can be significantly influenced by the host plant.
|
巻・号 |
13(2)
|
ページ |
411-5
|
公開日 |
2011-3-1
|
DOI |
10.1111/j.1438-8677.2010.00409.x
|
PMID |
21309988
|
MeSH |
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Dimethylallyltranstransferase / genetics
Dimethylallyltranstransferase / metabolism*
Flavanones / biosynthesis
Flavonoids / biosynthesis*
Fruit / chemistry
Fruit / genetics
Lycopersicon esculentum / chemistry*
Lycopersicon esculentum / genetics
Plants, Genetically Modified / enzymology
Plants, Genetically Modified / genetics
Prenylation
Promoter Regions, Genetic
RNA, Plant / genetics
Reverse Transcriptase Polymerase Chain Reaction
Streptomyces coelicolor / enzymology*
Streptomyces coelicolor / genetics
Tandem Mass Spectrometry
|
IF |
2.167
|
引用数 |
8
|
WOS 分野
|
PLANT SCIENCES
|
リソース情報 |
トマト |
TOMJPF00001 |