RRC ID 7183
著者 Shirasawa K, Isobe S, Hirakawa H, Asamizu E, Fukuoka H, Just D, Rothan C, Sasamoto S, Fujishiro T, Kishida Y, Kohara M, Tsuruoka H, Wada T, Nakamura Y, Sato S, Tabata S.
タイトル SNP discovery and linkage map construction in cultivated tomato.
ジャーナル DNA Res.
Abstract Few intraspecific genetic linkage maps have been reported for cultivated tomato, mainly because genetic diversity within Solanum lycopersicum is much less than that between tomato species. Single nucleotide polymorphisms (SNPs), the most abundant source of genomic variation, are the most promising source of polymorphisms for the construction of linkage maps for closely related intraspecific lines. In this study, we developed SNP markers based on expressed sequence tags for the construction of intraspecific linkage maps in tomato. Out of the 5607 SNP positions detected through in silico analysis, 1536 were selected for high-throughput genotyping of two mapping populations derived from crosses between 'Micro-Tom' and either 'Ailsa Craig' or 'M82'. A total of 1137 markers, including 793 out of the 1338 successfully genotyped SNPs, along with 344 simple sequence repeat and intronic polymorphism markers, were mapped onto two linkage maps, which covered 1467.8 and 1422.7 cM, respectively. The SNP markers developed were then screened against cultivated tomato lines in order to estimate the transferability of these SNPs to other breeding materials. The molecular markers and linkage maps represent a milestone in the genomics and genetics, and are the first step toward molecular breeding of cultivated tomato. Information on the DNA markers, linkage maps, and SNP genotypes for these tomato lines is available at http://www.kazusa.or.jp/tomato/.
巻・号 17(6)
ページ 381-91
公開日 2010-11-4
DOI 10.1093/dnares/dsq024
PII dsq024
PMID 21044984
PMC PMC2993540
MeSH Breeding Chromosome Mapping / methods* DNA, Plant / genetics Expressed Sequence Tags Genetic Markers Genetic Variation Genome, Plant* Introns Lycopersicon esculentum / genetics* Polymorphism, Single Nucleotide*
IF 5.404
引用数 53
トマト EST TOMJPF00004(Ailsa Craig) TOMJPF00003(Aichi First)