RRC ID 7184
Author Kato K, Yoshida R, Kikuzaki A, Hirai T, Kuroda H, Hiwasa-Tanase K, Takane K, Ezura H, Mizoguchi T.
Title Molecular breeding of tomato lines for mass production of miraculin in a plant factory.
Journal J Agric Food Chem
Abstract A transgenic tomato line (56B, "Moneymaker") that expresses the miraculin gene driven by the CaMV 35S promoter was crossed with a dwarf tomato ("Micro-Tom") for the molecular breeding of cultivars that are suitable for miraculin production in a closed cultivation system. Plant size, miraculin accumulation, and self-pruning growth were used as selection indicators for F2 plants. Two lines were chosen for further analysis, bred to the F6 or F7 generation and cultivated in a closed cultivation system. In 56B and the two crossed lines, the concentrations of miraculin in the pericarp were 140, 367, and 343 microg/g FW, respectively. We also estimated that 26.2, 73.6, and 45.9 kg FW/m2 of tomatoes and 2.2, 16.6, and 9.8 mg/m2 of miraculin in the pericarp, respectively, could be harvested per year. These two crossed lines will be useful for the mass production of miraculin, especially in a closed cultivation system.
Volume 58(17)
Pages 9505-10
Published 2010-9-8
DOI 10.1021/jf101874b
PMID 20695489
MeSH Enzyme-Linked Immunosorbent Assay Glycoproteins / genetics* Lycopersicon esculentum / genetics* Plants, Genetically Modified
IF 4.192
Times Cited 32
Tomato TOMJPF00002(Moneymaker)? TOMJPF00001?