Reference - Detail
| RRC ID | 7187 |
|---|---|
| Author | Hirai T, Fukukawa G, Kakuta H, Fukuda N, Ezura H. |
| Title | Production of recombinant miraculin using transgenic tomatoes in a closed cultivation system. |
| Journal | J Agric Food Chem |
| Abstract |
We constructed a cultivation system with a controlled light period, light intensity, temperature, and CO(2) concentration for mass production of the taste-modifying protein miraculin from transgenic tomatoes. The tomato plants exhibited normal growth and produced over 270 g of fresh weight (FW) fruit per plant, with the recombinant miraculin concentration reaching up to 90 microg per g FW of tomatoes. The recombinant miraculin content of transgenic tomatoes was compared to that of plants grown in a netted greenhouse. The recombinant miraculin content of transgenic tomatoes grown in a closed cultivation system was more stable than that of tomatoes grown in a netted greenhouse, suggesting that the closed cultivation system is suitable for the production of recombinant miraculin. We estimate that 45 tFW of tomatoes and 4 kg of recombinant miraculin per 1,000 m(2) of cultivation area can be harvested per year. |
| Volume | 58(10) |
| Pages | 6096-101 |
| Published | 2010-5-26 |
| DOI | 10.1021/jf100414v |
| PMID | 20426470 |
| MeSH | Agriculture / methods Environment, Controlled Fruit / chemistry Fruit / metabolism Glycoproteins / analysis Glycoproteins / biosynthesis* Glycoproteins / genetics Lycopersicon esculentum / genetics Lycopersicon esculentum / growth & development* Lycopersicon esculentum / metabolism* Plants, Genetically Modified / metabolism* Recombinant Proteins / analysis Recombinant Proteins / biosynthesis* |
| IF | 4.192 |
| Times Cited | 21 |
| WOS Category | AGRICULTURE, MULTIDISCIPLINARY FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Tomato | TOMJPF00002(Moneymaker) |