論文 - 詳細
| RRC ID | 71897 |
|---|---|
| 著者 | Nakagami A, Mao Q, Gouhier G, Arima H, Kitagishi H. |
| タイトル | FRET-Based In-Cell Detection of Highly Selective Supramolecular Complexes of meso-Tetraarylporphyrin with Peptide/BODIPY-Modified Per-O-Methyl-β-Cyclodextrins. |
| ジャーナル | Chembiochem |
| Abstract |
Artificial supramolecular systems capable of self-assembly and that precisely function in biological media are in high demand. Herein, we demonstrate a highly specific host-guest-pair system that functions in living cells. A per-O-methyl-β-cyclodextrin derivative (R8-B-CDMe ) bearing both an octaarginine peptide chain and a BODIPY dye was synthesized as a fluorescent intracellular delivery tool. R8-B-CDMe was efficiently taken up by HeLa cells through both endocytosis and direct transmembrane pathways. R8-B-CDMe formed a 2 : 1 inclusion complex with tetrakis(4-sulfonatophenyl)porphyrin (TPPS) as a guest molecule in water, from which fluorescence resonance energy transfer (FRET) from R8-B-CDMe to TPPS was observed. The FRET phenomenon was clearly detected in living cells using confocal microscopy techniques, which revealed that the formed supramolecular R8-B-CDMe /TPPS complex was maintained within the cells. The R8-B-CDMe cytotoxicity assay revealed that the addition of TPPS counteracts the strong cytotoxicity (IC50 =16 μM) of the CD cavity due to complexation within the cells. A series of experiments demonstrated the bio-orthogonality of the supramolecular per-O-methyl-β-CD/tetraarylporphyrin host-guest pair in living cells. |
| 巻・号 | 22(22) |
| ページ | 3190-3198 |
| 公開日 | 2021-11-16 |
| DOI | 10.1002/cbic.202100380 |
| PMID | 34467611 |
| MeSH | Boron Compounds / chemistry* Fluorescence Resonance Energy Transfer* HeLa Cells Humans Macromolecular Substances / chemistry Mesoporphyrins / chemistry* Molecular Structure Peptides / chemistry* Spectrometry, Fluorescence beta-Cyclodextrins / chemistry* |
| IF | 2.576 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |