論文 - 詳細
| RRC ID | 7190 |
|---|---|
| 著者 | Hiwasa-Tanase K, Nyarubona M, Hirai T, Kato K, Ichikawa T, Ezura H. |
| タイトル | High-level accumulation of recombinant miraculin protein in transgenic tomatoes expressing a synthetic miraculin gene with optimized codon usage terminated by the native miraculin terminator. |
| ジャーナル | Plant Cell Rep |
| Abstract |
In our previous study, a transgenic tomato line that expressed the MIR gene under control of the cauliflower mosaic virus 35S promoter and the nopaline synthase terminator (tNOS) produced the taste-modifying protein miraculin (MIR). However, the concentration of MIR in the tomatoes was lower than that in the MIR gene's native miracle fruit. To increase MIR production, the native MIR terminator (tMIR) was used and a synthetic gene encoding MIR protein (sMIR) was designed to optimize its codon usage for tomato. Four different combinations of these genes and terminators (MIR-tNOS, MIR-tMIR, sMIR-tNOS and sMIR-tMIR) were constructed and used for transformation. The average MIR concentrations in MIR-tNOS, MIR-tMIR, sMIR-tNOS and sMIR-tMIR fruits were 131, 197, 128 and 287 μg/g fresh weight, respectively. The MIR concentrations using tMIR were higher than those using tNOS. The highest MIR accumulation was detected in sMIR-tMIR fruits. On the other hand, the MIR concentration was largely unaffected by sMIR-tNOS. The expression levels of both MIR and sMIR mRNAs terminated by tMIR tended to be higher than those terminated by tNOS. Read-through mRNA transcripts terminated by tNOS were much longer than those terminated by tMIR. These results suggest that tMIR enhances mRNA expression and permits the multiplier effect of optimized codon usage. |
| 巻・号 | 30(1) |
| ページ | 113-24 |
| 公開日 | 2011-1-1 |
| DOI | 10.1007/s00299-010-0949-y |
| PMID | 21076835 |
| MeSH | Base Sequence Blotting, Southern Codon / genetics* Fruit / genetics Gene Expression Regulation, Plant Genes, Plant / genetics* Glucuronidase / metabolism Glycoproteins / genetics Glycoproteins / metabolism* Lycopersicon esculentum / genetics* Lycopersicon esculentum / metabolism* Molecular Sequence Data Plants, Genetically Modified Polyadenylation / genetics Recombinant Proteins / metabolism* Terminator Regions, Genetic / genetics* Transcription, Genetic Transgenes / genetics |
| IF | 3.825 |
| 引用数 | 16 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| トマト | TOMJPF00001 |