論文 - 詳細
| RRC ID | 71907 |
|---|---|
| 著者 | Takizawa Y, Nakamura R, Kurita T, Nakajima T. |
| タイトル | Lactose hydrate can increase the transcellular permeability of β-naphthol in rat jejunum and ileum. |
| ジャーナル | Mol Biol Rep |
| Abstract |
BACKGROUND:The unstirred water layer (UWL) is an integral part of the apical surface of mucosal epithelia and comprises mucins (MUC), for which there are many molecular species. Galectins, a family of β-galactoside-binding lectins, form a lattice barrier on surface epithelial cells by interacting with MUC. Lactose inhibits the galectin-MUC interaction. Therefore, the present study investigated the galectin-MUC interaction in the mucosa of the gastrointestinal tract and its role in intestinal barrier functions. MATERIALS AND RESULTS:The effects of lactose hydrate (LH) on the membrane permeability of the rat small intestine and Caco-2 cells were examined. LH enhanced the membrane permeability of the rat small intestine, which contains the UWL, via a transcellular route, for which the UWL is the rate limiting factor. The membrane permeability of Caco-2 cells, in which the UWL is insufficient, was not affected by LH. The apparent permeability coefficient (Papp) of a paracellular marker was not significantly altered in the rat small intestine or Caco-2 cells treated with LH at any concentration. Furthermore, the Papp of β-naphthol which is a transcellular marker was not significantly altered in Caco-2 cells treated with LH, but was significantly increased in the rat small intestine in a LH concentration-dependent manner. CONCLUSIONS:The present results demonstrate that the physical barrier has an important function in gastrointestinal membrane permeability, and LH-induced changes increase the transcellular permeability of β-naphthol in rat small intestine. |
| 巻・号 | 49(9) |
| ページ | 8685-8692 |
| 公開日 | 2022-9-1 |
| DOI | 10.1007/s11033-022-07709-2 |
| PII | 10.1007/s11033-022-07709-2 |
| PMID | 35767107 |
| MeSH | Animals Caco-2 Cells Galectins Humans Ileum / metabolism Intestinal Absorption* Intestinal Mucosa / metabolism Jejunum* / metabolism Lactose / metabolism Lactose / pharmacology Naphthols Permeability Rats Water / chemistry |
| IF | 1.402 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |