論文 - 詳細
| RRC ID | 71925 |
|---|---|
| 著者 | Oikawa T, Fukuda T, Yamashita T, Tomita H, Ozaki T. |
| タイトル | Lentiviral expression of calpain-1 C2-like domain peptide prevents glutamate-induced cell death in mouse hippocampal neuronal HT22 cells. |
| ジャーナル | In Vitro Cell Dev Biol Anim |
| Abstract |
Glutamate neurotoxicity is involved in neurodegenerative diseases, including Alzheimer's and Parkinson's diseases. Excess glutamate causes caspase-independent programmed cell death via oxidative stress and calcium influx. Our previous study showed that calpain-1 localizes to both the cytoplasm and mitochondria, where apoptosis-inducing factor (AIF) is cleaved by calpain-1 and translocates to the nucleus to induce DNA fragmentation. The autoinhibitory region of calpain-1 conjugated with the cell-penetrating peptide HIV1-Tat (namely Tat-μCL) specifically prevents the activity of mitochondrial calpain-1 and attenuates neuronal cell death in animal models of retinitis pigmentosa, as well as glutamate-induced cell death in mouse hippocampal HT22 cells. In the present study, we constructed a lentiviral vector expressing the Tat-μCL peptide and evaluated its protective effect against glutamate-induced cell death in HT22 cells. Lentiviral transduction with Tat-μCL significantly suppressed glutamate-induced nuclear translocation of AIF and DNA fragmentation. The findings of the present study suggest that the stable expression of Tat-μCL may be a potential gene therapy modality for neurodegenerative diseases. |
| 巻・号 | 58(4) |
| ページ | 289-294 |
| 公開日 | 2022-4-1 |
| DOI | 10.1007/s11626-022-00683-w |
| PII | 10.1007/s11626-022-00683-w |
| PMID | 35469046 |
| MeSH | Animals Apoptosis Inducing Factor / genetics Apoptosis Inducing Factor / metabolism Calpain* / genetics Calpain* / metabolism Cell Death Glutamic Acid* / metabolism Glutamic Acid* / toxicity Hippocampus / metabolism Mice Oxidative Stress Peptides / metabolism |
| IF | 1.665 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393) |