RRC ID 71934
Author Watanabe K, Nawachi T, Okutani R, Ohtsuki T.
Title Photocontrolled apoptosis induction using precursor miR-664a and an RNA carrier-conjugated with photosensitizer.
Journal Sci Rep
Abstract Methods to spatially induce apoptosis are useful for cancer therapy. To control the induction of apoptosis, methods using light, such as photochemical internalization (PCI), have been developed. We hypothesized that photoinduced delivery of microRNAs (miRNAs) that regulate apoptosis could spatially induce apoptosis. In this study, we identified pre-miR-664a as a novel apoptosis-inducing miRNA via mitochondrial apoptotic pathway. Further, we demonstrated the utility of photoinduced cytosolic dispersion of RNA (PCDR), which is an intracellular RNA delivery method based on PCI. Indeed, apoptosis is spatially regulated by pre-miR-664a and PCDR. In addition, we found that apoptosis induced by pre-miR-664a delivered by PCDR was more rapid than that by lipofection. These results suggest that pre-miR-664a is a nucleic acid drug candidate for cancer therapy and PCDR and pre-miR-664a-based strategies have potential therapeutic uses for diseases affecting various cell types.
Volume 11(1)
Pages 14936
Published 2021-7-22
DOI 10.1038/s41598-021-94249-7
PII 10.1038/s41598-021-94249-7
PMID 34294789
PMC PMC8298592
MeSH Cell Line, Tumor Cell Proliferation / drug effects Cell Survival / drug effects Cytosol / chemistry HeLa Cells Humans MicroRNAs / chemistry MicroRNAs / pharmacology* Neoplasms / drug therapy Neoplasms / genetics* Photosensitizing Agents / chemistry* Quinolinium Compounds / chemistry* Ribonucleoprotein, U1 Small Nuclear / chemistry Ribonucleoprotein, U1 Small Nuclear / genetics Transfection
Human and Animal Cells HeLa