| RRC ID |
71963
|
| 著者 |
Hori K, Higashida S, Osaki T, Kawano T, Inaba H, Matsuura K, Iwasaki T.
|
| タイトル |
Intracellular delivery and photothermal therapeutic effects of polyhistidine peptide-modified gold nanoparticles.
|
| ジャーナル |
J Biotechnol
|
| Abstract |
Gold nanoparticles (AuNPs) are widely used as an agent in photothermal therapy (PTT) against various cancers. However, a drug delivery system (DDS) is required for effective PTT using AuNPs as AuNPs accumulate passively in tumors. In the present study, we used polyhistidine peptide, a novel cell-penetrating peptide, which is efficiently internalized into tumor cells, as a DDS carrier for PTT using AuNPs. Polyhistidine peptide-modified AuNPs are efficiently internalized into RERF-LC-AI human lung squamous cancer cells and localized to the intracellular lysosome, which is based on the nature of the polyhistidine peptide. Furthermore, the polyhistidine peptide-modified AuNPs inhibited proliferation of RERF-LC-AI cells in a polyhistidine peptide modification-dependent manner under 660 nm laser irradiation. Quantitative real-time PCR showed increased expression levels of an apoptosis-related gene (bax) and heat stress-related gene (hsp70) in RERF-LC-AI cells treated with polyhistidine peptide-modified AuNPs and laser. Our findings highlight the efficacy of AuNPs modified with H16 peptide in PTT.
|
| 巻・号 |
354
|
| ページ |
34-44
|
| 公開日 |
2022-8-10
|
| DOI |
10.1016/j.jbiotec.2022.06.006
|
| PII |
S0168-1656(22)00144-4
|
| PMID |
35724765
|
| MeSH |
Cell Line, Tumor
Cell-Penetrating Peptides* / pharmacology
Gold / pharmacology
Histidine* / pharmacology
Humans
Metal Nanoparticles* / therapeutic use
Phototherapy / methods
|
| IF |
3.503
|
| リソース情報 |
| ヒト・動物細胞 |
Hep G2(RCB1886)
RERF-LC-AI(RCB0444)
HT1080(RCB2102)
CACO-2(RCB0988) |
