論文 - 詳細
| RRC ID | 71963 |
|---|---|
| 著者 | Hori K, Higashida S, Osaki T, Kawano T, Inaba H, Matsuura K, Iwasaki T. |
| タイトル | Intracellular delivery and photothermal therapeutic effects of polyhistidine peptide-modified gold nanoparticles. |
| ジャーナル | J Biotechnol |
| Abstract |
Gold nanoparticles (AuNPs) are widely used as an agent in photothermal therapy (PTT) against various cancers. However, a drug delivery system (DDS) is required for effective PTT using AuNPs as AuNPs accumulate passively in tumors. In the present study, we used polyhistidine peptide, a novel cell-penetrating peptide, which is efficiently internalized into tumor cells, as a DDS carrier for PTT using AuNPs. Polyhistidine peptide-modified AuNPs are efficiently internalized into RERF-LC-AI human lung squamous cancer cells and localized to the intracellular lysosome, which is based on the nature of the polyhistidine peptide. Furthermore, the polyhistidine peptide-modified AuNPs inhibited proliferation of RERF-LC-AI cells in a polyhistidine peptide modification-dependent manner under 660 nm laser irradiation. Quantitative real-time PCR showed increased expression levels of an apoptosis-related gene (bax) and heat stress-related gene (hsp70) in RERF-LC-AI cells treated with polyhistidine peptide-modified AuNPs and laser. Our findings highlight the efficacy of AuNPs modified with H16 peptide in PTT. |
| 巻・号 | 354 |
| ページ | 34-44 |
| 公開日 | 2022-8-10 |
| DOI | 10.1016/j.jbiotec.2022.06.006 |
| PII | S0168-1656(22)00144-4 |
| PMID | 35724765 |
| MeSH | Cell Line, Tumor Cell-Penetrating Peptides* / pharmacology Gold / pharmacology Histidine* / pharmacology Humans Metal Nanoparticles* / therapeutic use Phototherapy / methods |
| IF | 3.503 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB1886) RERF-LC-AI(RCB0444) HT1080(RCB2102) CACO-2(RCB0988) |