論文 - 詳細
| RRC ID | 71967 |
|---|---|
| 著者 | Ogo A, Miyake S, Kubota H, Higashida M, Matsumoto H, Teramoto F, Hirai T, Ueno T. |
| タイトル | The Mechanism of the Synergistic Anticancer Effect of CDDP and EPA in the TE1 Cell Line. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:Eicosapentaenoic acid (EPA) is an unsaturated fatty acid with various bioactivities, including antitumor effects. We previously reported a synergistic antitumor effect of cisplatin (CDDP) and EPA. Here, we examined the underlying mechanism. MATERIALS AND METHODS:The human oesophageal cancer cell line TE-1 was treated with the combination of EPA and CDDP. Nuclear translocation of NF-κB, a transcription factor involved in cytokine production, was detected by immunohistochemistry. IL-6 levels were measured by ELISA. Apoptosis and cell cycle distribution were evaluated by flow cytometry. RESULTS:Nuclear translocation of NF-κB in TE-1 cells was synergistically decreased by CDDP and EPA. IL-6 production was increased following treatment with CDDP, but treatment with EPA decreased IL-6 levels. Apoptosis was synergistically induced by CDDP and EPA. A G2/M cell cycle arrest was observed with the combination of CDDP and 150 μM EPA, and S phase arrest with the combination of CDDP and 100 μM EPA. CONCLUSION:The combination of CDDP and EPA synergistically suppresses NF-κB nuclear translocation and increases apoptosis by inducing cell cycle arrest at the S or G2/M phase. |
| 巻・号 | 41(4) |
| ページ | 1771-1778 |
| 公開日 | 2021-4-1 |
| DOI | 10.21873/anticanres.14942 |
| PII | 41/4/1771 |
| PMID | 33813381 |
| MeSH | Antineoplastic Combined Chemotherapy Protocols / pharmacology* Apoptosis / drug effects Cell Line, Tumor Cisplatin / pharmacology* Drug Synergism Eicosapentaenoic Acid / pharmacology* Esophageal Neoplasms / drug therapy* Esophageal Neoplasms / metabolism Esophageal Neoplasms / pathology G2 Phase Cell Cycle Checkpoints / drug effects Humans Interleukin-6 / metabolism NF-kappa B / metabolism S Phase Cell Cycle Checkpoints / drug effects Signal Transduction |
| IF | 1.994 |
| リソース情報 | |
| ヒト・動物細胞 | TE-1(RCB1894) |