論文 - 詳細
| RRC ID | 71979 |
|---|---|
| 著者 | Shimohata N, Harada Y, Hayano T. |
| タイトル | Proteomic analysis of nascent polypeptide chains that potentially induce translational pausing during elongation. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
Currently, proteins equipped with "ribosomal arrest peptides" (RAPs) that regulate the expression of downstream genes and their own activity by pausing their own translation during elongation are extensively studied. However, studies focusing on RAP have been conducted primarily in prokaryotic cells; studies on eukaryotic cells, especially mammalian cells, are limited. In the present study, we comprehensively examined translationally arrested nascent polypeptides to gain novel insights into RAPs in mammalian cells. Cetyltrimethylammonium bromide was used to obtain nascent polypeptide chains that were translationally arrested during translation elongation. After proteomic analysis, additional screening by discriminating according to amino acid residues at the C-terminal end revealed several novel RAP candidates. Our method can be applied for comprehensive RAP studies in mammalian cells. |
| 巻・号 | 86(9) |
| ページ | 1262-1269 |
| 公開日 | 2022-8-24 |
| DOI | 10.1093/bbb/zbac097 |
| PII | 6617584 |
| PMID | 35749475 |
| MeSH | Animals Mammals / genetics Mammals / metabolism Peptide Chain Elongation, Translational* / physiology Peptides / chemistry Protein Biosynthesis Proteomics* Ribosomes / metabolism |
| IF | 1.516 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 10T1/2(RCB0247) ATDC5(RCB0565) |