RRC ID 71993
Author Jiang Y, Chen B, Duan C, Sun B, Yang J, Yang S.
Title Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system.
Journal Appl Environ Microbiol
Abstract An efficient genome-scale editing tool is required for construction of industrially useful microbes. We describe a targeted, continual multigene editing strategy that was applied to the Escherichia coli genome by using the Streptococcus pyogenes type II CRISPR-Cas9 system to realize a variety of precise genome modifications, including gene deletion and insertion, with a highest efficiency of 100%, which was able to achieve simultaneous multigene editing of up to three targets. The system also demonstrated successful targeted chromosomal deletions in Tatumella citrea, another species of the Enterobacteriaceae, with highest efficiency of 100%.
Volume 81(7)
Pages 2506-14
Published 2015-4-1
DOI 10.1128/AEM.04023-14
PII AEM.04023-14
PMID 25636838
PMC PMC4357945
MeSH CRISPR-Cas Systems* Enterobacteriaceae / enzymology Enterobacteriaceae / genetics* Genes, Bacterial Genetics, Microbial / methods* Genome, Bacterial Molecular Biology / methods* Recombination, Genetic Streptococcus pyogenes / enzymology Streptococcus pyogenes / genetics
IF 4.016
Prokaryotes E. coli pCas