RRC ID 720
Author Oguchi T, Sage-Ono K, Kamada H, Ono M.
Title Characterization of transcriptional oscillation of an Arabidopsis homolog of PnC401 related to photoperiodic induction of flowering in Pharbitis nil.
Journal Plant Cell Physiol.
Abstract AtC401 is an Arabidopsis homolog of PnC401 that is related to photoperiodic induction of flowering in Pharbitis nil. These genes show free-running rhythms. To study the free-running rhythm of AtC401, we fused a firefly luciferase reporter to the AtC401 promoter and transformed it into Arabidopsis plants. The observed bioluminescence oscillated under continuous light and continuous dark only with sucrose supplementation. The free-running period of bioluminescence was temperature-compensated between 22 degrees C and 30 degrees C. Light-pulse experiments under continuous darkness produced a phase-response curve typical of circadian rhythms. We conclude that rhythmic expression of AtC401 is controlled by a circadian oscillator.
Volume 45(2)
Pages 232-5
Published 2004-2
PMID 14988494
MeSH Arabidopsis / genetics* Arabidopsis / growth & development Arabidopsis / radiation effects Arabidopsis Proteins / genetics* Biological Clocks / genetics* Circadian Rhythm / genetics Flowers / genetics* Flowers / growth & development Flowers / radiation effects Gene Expression Regulation, Plant / genetics Genes, Regulator / genetics* Genes, Reporter / genetics Ipomoea / genetics* Luciferases / genetics Luminescent Measurements Photic Stimulation Photoperiod Plants, Genetically Modified / genetics Plants, Genetically Modified / growth & development Plants, Genetically Modified / radiation effects
IF 4.059
Times Cited 0
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