論文 - 詳細
| RRC ID | 72015 |
|---|---|
| 著者 | Kanatsu-Shinohara M, Lee J, Miyazaki T, Morimoto H, Shinohara T. |
| タイトル | Adeno-associated-virus-mediated gene delivery to ovaries restores fertility in congenital infertile mice. |
| ジャーナル | Cell Rep Med |
| Abstract |
Oocytes and granulosa cells closely interact with each other during follicular development, and a lack of appropriate signaling between them results in infertility. Attempts to manipulate oocyte microenvironment have been impeded by the impermeability of the blood-follicle barrier (BFB). To establish a strategy for manipulating oogenesis, we use adeno-associated viruses (AAVs), which have a unique ability of transcytosis. Microinjecting of AAVs into the ovarian stroma penetrates the BFB and achieves long-term gene expression. Introduction of an AAV carrying the mouse Kitl gene restores oogenesis in congenitally infertile KitlSl-t/KitlSl-t mutant mouse ovaries, which lack Kitl expression but contain only primordial follicles. Healthy offspring without AAV integration are born by natural mating. Therefore, AAV-mediated gene delivery not only provides a means for studying oocyte-granulosa interactions through the manipulation of the oocyte microenvironment but could also be a powerful method to treat female infertility resulting from somatic cell defects. |
| 巻・号 | 3(5) |
| ページ | 100606 |
| 公開日 | 2022-5-17 |
| DOI | 10.1016/j.xcrm.2022.100606 |
| PII | S2666-3791(22)00123-9 |
| PMID | 35584625 |
| PMC | PMC9133397 |
| MeSH | Animals Dependovirus / genetics Female Fertility / genetics Humans Infertility, Female* / genetics Mice Ovarian Follicle Ovary* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 15 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | CSII-EF-MCS (RDB04378) CSII-EF-MCS-IRES2-Venus (RDB04384) pAxcw (RDB00917) |