| RRC ID |
72044
|
| 著者 |
Suzuki T, Sato Y, Okuno Y, Goshima F, Mikami T, Umeda M, Murata T, Watanabe T, Watashi K, Wakita T, Kitagawa H, Kimura H.
|
| タイトル |
Genome-wide CRISPR screen for HSV-1 host factors reveals PAPSS1 contributes to heparan sulfate synthesis.
|
| ジャーナル |
Commun Biol
|
| Abstract |
Herpes simplex virus type 1 (HSV-1) is a ubiquitous pathogen that causes various diseases in humans, ranging from common mucocutaneous lesions to severe life-threatening encephalitis. However, our understanding of the interaction between HSV-1 and human host factors remains incomplete. Here, to identify the host factors for HSV-1 infection, we performed a human genome-wide CRISPR screen using near-haploid HAP1 cells, in which gene knockout (KO) could be efficiently achieved. Along with several already known host factors, we identified 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1) as a host factor for HSV-1 infection. The KO of PAPSS1 in HAP1 cells reduced heparan sulfate (HepS) expression, consequently diminishing the binding of HSV-1 and several other HepS-dependent viruses (such as HSV-2, hepatitis B virus, and a human seasonal coronavirus). Hence, our findings provide further insights into the host factor requirements for HSV-1 infection and HepS biosynthesis.
|
| 巻・号 |
5(1)
|
| ページ |
694
|
| 公開日 |
2022-7-19
|
| DOI |
10.1038/s42003-022-03581-9
|
| PII |
10.1038/s42003-022-03581-9
|
| PMID |
35854076
|
| PMC |
PMC9296583
|
| MeSH |
Clustered Regularly Interspaced Short Palindromic Repeats
Gene Knockout Techniques
Heparitin Sulfate / metabolism
Herpes Simplex* / genetics
Herpesvirus 1, Human* / genetics
Humans
|
| IF |
4.165
|
| リソース情報 |
| ヒト・動物細胞 |
HuH-7(RCB1942) |
| 遺伝子材料 |
CSII-CMV-MCS-IRES2-Bsd (RDB04385) |
