論文 - 詳細
| RRC ID | 72044 |
|---|---|
| 著者 | Suzuki T, Sato Y, Okuno Y, Goshima F, Mikami T, Umeda M, Murata T, Watanabe T, Watashi K, Wakita T, Kitagawa H, Kimura H. |
| タイトル | Genome-wide CRISPR screen for HSV-1 host factors reveals PAPSS1 contributes to heparan sulfate synthesis. |
| ジャーナル | Commun Biol |
| Abstract |
Herpes simplex virus type 1 (HSV-1) is a ubiquitous pathogen that causes various diseases in humans, ranging from common mucocutaneous lesions to severe life-threatening encephalitis. However, our understanding of the interaction between HSV-1 and human host factors remains incomplete. Here, to identify the host factors for HSV-1 infection, we performed a human genome-wide CRISPR screen using near-haploid HAP1 cells, in which gene knockout (KO) could be efficiently achieved. Along with several already known host factors, we identified 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1) as a host factor for HSV-1 infection. The KO of PAPSS1 in HAP1 cells reduced heparan sulfate (HepS) expression, consequently diminishing the binding of HSV-1 and several other HepS-dependent viruses (such as HSV-2, hepatitis B virus, and a human seasonal coronavirus). Hence, our findings provide further insights into the host factor requirements for HSV-1 infection and HepS biosynthesis. |
| 巻・号 | 5(1) |
| ページ | 694 |
| 公開日 | 2022-7-19 |
| DOI | 10.1038/s42003-022-03581-9 |
| PII | 10.1038/s42003-022-03581-9 |
| PMID | 35854076 |
| PMC | PMC9296583 |
| MeSH | Clustered Regularly Interspaced Short Palindromic Repeats Gene Knockout Techniques Heparitin Sulfate / metabolism Herpes Simplex* / genetics Herpesvirus 1, Human* / genetics Humans |
| IF | 4.165 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HuH-7(RCB1942) |
| 遺伝子材料 | CSII-CMV-MCS-IRES2-Bsd (RDB04385) |