論文 - 詳細
| RRC ID | 72045 |
|---|---|
| 著者 | Sim EZ, Enomoto T, Shiraki N, Furuta N, Kashio S, Kambe T, Tsuyama T, Arakawa A, Ozawa H, Yokoyama M, Miura M, Kume S. |
| タイトル | Methionine metabolism regulates pluripotent stem cell pluripotency and differentiation through zinc mobilization. |
| ジャーナル | Cell Rep |
| Abstract |
Pluripotent stem cells (PSCs) exhibit a unique feature that requires S-adenosylmethionine (SAM) for the maintenance of their pluripotency. Methionine deprivation in the medium causes a reduction in intracellular SAM, thus rendering PSCs in a state potentiated for differentiation. In this study, we find that methionine deprivation triggers a reduction in intracellular protein-bound Zn content and upregulation of Zn exporter SLC30A1 in PSCs. Culturing PSCs in Zn-deprived medium results in decreased intracellular protein-bound Zn content, reduced cell growth, and potentiated differentiation, which partially mimics methionine deprivation. PSCs cultured under Zn deprivation exhibit an altered methionine metabolism-related metabolite profile. We conclude that methionine deprivation potentiates differentiation partly by lowering cellular Zn content. We establish a protocol to generate functional pancreatic β cells by applying methionine and Zn deprivation. Our results reveal a link between Zn signaling and methionine metabolism in the regulation of cell fate in PSCs. |
| 巻・号 | 40(3) |
| ページ | 111120 |
| 公開日 | 2022-7-19 |
| DOI | 10.1016/j.celrep.2022.111120 |
| PII | S2211-1247(22)00926-3 |
| PMID | 35858556 |
| MeSH | Cell Differentiation / physiology Methionine / metabolism Pluripotent Stem Cells* / metabolism S-Adenosylmethionine / metabolism Signal Transduction Zinc* / metabolism |
| IF | 8.109 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 17 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) |