Reference - Detail
| RRC ID | 72103 |
|---|---|
| Author | Miyoshi T, Friedman TB, Watanabe N. |
| Title | Fast-dissociating but highly specific antibodies are novel tools in biology, especially useful for multiplex super-resolution microscopy. |
| Journal | STAR Protoc |
| Abstract |
Fast-dissociating, highly specific monoclonal antibodies (FDSAs) are single-molecule imaging probes useful for many biological assays including consecutive, multiplexable super-resolution microscopy. We developed a screening assay to characterize the kinetics of antibody-antigen interactions using single-molecule microscopy and established a pipeline to identify FDSAs from thousands of monoclonal candidates. Provided here are detailed protocols to prepare multi-well glass-bottom plates necessary for our assay to identify hybridoma clones secreting FDSAs. Synthesis of fluorescently labeled Fab fragments (Fab probes) from FDSAs is also described. For complete details on the use and execution of this protocol, please refer to Miyoshi et al. (2021). |
| Volume | 2(4) |
| Pages | 100967 |
| Published | 2021-12-17 |
| DOI | 10.1016/j.xpro.2021.100967 |
| PII | S2666-1667(21)00673-0 |
| PMID | 34841279 |
| PMC | PMC8605432 |
| MeSH | Animals Antibodies, Monoclonal* / chemistry Antibodies, Monoclonal* / metabolism Female Fluorescent Dyes* / chemistry Fluorescent Dyes* / metabolism Hybridomas / chemistry Hybridomas / metabolism Immunoglobulin Fab Fragments / chemistry Immunoglobulin Fab Fragments / metabolism Mice Mice, Inbred BALB C Microscopy, Fluorescence / methods* Single Molecule Imaging / methods* |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | UV-P3U1-B8(RCB1157) |