論文 - 詳細
| RRC ID | 72174 |
|---|---|
| 著者 | Takahashi K, Orito N, Yanagisawa D, Yano A, Mori Y, Inoue N. |
| タイトル | Eosinophils are the main cellular targets for oral gene delivery using Lactic acid bacteria. |
| ジャーナル | Vaccine |
| Abstract |
Lactic acid bacteria have been studied as a vehicle for the delivery of plasmid DNA to the gastrointestinal tract. However, low levels of gene expression in vivo limit their practical use. Furthermore, it is still unclear how the orally administrated bacteria transfer their harbored plasmid DNA to host intestinal cells. To more easily track the delivery of plasmid DNA for eukaryotic expression in the intestine, we constructed an L. lactis-E. coli shuttle plasmid (pLEC) that allowed significantly elevated expression of the target protein of interest in eukaryotic cells. We first demonstrated its usefulness for delivery from L. lactis to Caco-2 cells in vitro. We then investigated the cellular target for the L. lactis DNA delivery system in vivo. Mice were orally administrated with LL/pLEC:EGFP, an L. lactis strain carrying pLEC for EGFP expression, and immunofluorescent analyses of frozen sections prepared from their small intestines identified a number of EGFP-expressing cells in the lamina propria and some in the sub-epithelial dome of the Peyer's patches. Flow cytometric analysis revealed that these EGFP-expressing cells were both CD11c- and F4/80-positive but CX3CR1-negative, suggesting that they are eosinophils. Immunostaining of the sections with an antibody against Siglec-F, a marker protein of eosinophils, confirmed the flow cytometric findings. Thus, the target cells of DNA delivery from L. lactis in the intestines are mainly eosinophils in the lamina propria and Peyer's patches. This finding may open a new approach to the development of DNA vaccines for oral administration. |
| 巻・号 | 38(17) |
| ページ | 3330-3338 |
| 公開日 | 2020-4-9 |
| DOI | 10.1016/j.vaccine.2020.02.084 |
| PII | S0264-410X(20)30329-7 |
| PMID | 32197922 |
| MeSH | Administration, Oral Animals Caco-2 Cells Eosinophils* Escherichia coli / genetics Genetic Vectors* Green Fluorescent Proteins / genetics Humans Lactococcus lactis* Mice Plasmids / genetics* |
| IF | 3.143 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |