Reference - Detail
| RRC ID | 72212 |
|---|---|
| Author | Nittayaboon K, Leetanaporn K, Sangkhathat S, Roytrakul S, Navakanitworakul R. |
| Title | Cytotoxic effect of metformin on butyrate-resistant PMF-K014 colorectal cancer spheroid cells. |
| Journal | Biomed Pharmacother |
| Abstract |
Three-dimensional (3D) cell culture models are used in cancer research because they mimic physiological responses in vivo compared with two-dimensional (2D) culture systems. Recently, cross-resistance of butyrate-resistant (BR) cells and chemoresistance in colorectal cancer (CRC) cells have been reported; however, effective treatments for BR cells have not been identified. In this study, we investigated the cytotoxicity of metformin (MET), an anti-diabetic drug, on BR CRC cells in a 3D spheroid culture model. The results demonstrate that MET decreases spheroid size, migration, and spheroid viability, while it increases spheroid death. The molecular mechanism revealed that AMP-activated protein kinase (AMPK) and Akt serine/threonine kinase 1(Akt) were significantly upregulated, whereas the acetyl-CoA-carboxylase (ACC) and mammalian target of rapamycin (mTOR) were downregulated, which led to caspase activation and apoptosis. Our findings show the potential cytotoxicity of MET on CRC-BR cells. The combination of MET and conventional chemotherapeutic drugs should be addressed in further studies to reduce the side effects of standard chemotherapy for CRC. |
| Volume | 151 |
| Pages | 113214 |
| Published | 2022-7-1 |
| DOI | 10.1016/j.biopha.2022.113214 |
| PII | S0753-3322(22)00603-5 |
| PMID | 35676792 |
| MeSH | AMP-Activated Protein Kinases / metabolism Antineoplastic Agents* / pharmacology Antineoplastic Agents* / therapeutic use Butyrates / pharmacology Butyrates / therapeutic use Cell Line, Tumor Colorectal Neoplasms* / drug therapy Humans Metformin* / pharmacology Metformin* / therapeutic use Proto-Oncogene Proteins c-akt / metabolism |
| IF | 4.545 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | PMF-ko14(RCB1426) |