RRC ID 72229
著者 Lee KH, Chun Y, Lee JH, Park C, Yoo HY, Kwak HS.
タイトル Improved Productivity of Astaxanthin from Photosensitive Haematococcus pluvialis Using Phototaxis Technology.
ジャーナル Mar Drugs
Abstract Haematococcus pluvialis is a microalgae actively studied for the production of natural astaxanthin, which is a powerful antioxidant for human application. However, it is economically disadvantageous for commercialization owing to the low productivity of astaxanthin. This study reports an effective screening strategy using the negative phototaxis of the H. pluvialis to attain the mutants having high astaxanthin production. A polydimethylsiloxane (PDMS)-based microfluidic device irradiated with a specific light was developed to efficiently figure out the phototactic response of H. pluvialis. The partial photosynthesis deficient (PP) mutant (negative control) showed a 0.78-fold decreased cellular response to blue light compared to the wild type, demonstrating the positive relationship between the photosynthetic efficiency and the phototaxis. Based on this relationship, the Haematococcus mutants showing photosensitivity to blue light were selected from the 10,000 random mutant libraries. The M1 strain attained from the phototaxis-based screening showed 1.17-fold improved growth rate and 1.26-fold increases in astaxanthin production (55.12 ± 4.12 mg g-1) in the 100 L photo-bioreactor compared to the wild type. This study provides an effective selection tool for industrial application of the H. pluvialis with improved astaxanthin productivity.
巻・号 20(4)
公開日 2022-3-22
DOI 10.3390/md20040220
PII md20040220
PMID 35447893
PMC PMC9032356
MeSH Bioreactors Chlorophyceae* Chlorophyta* Humans Phototaxis Xanthophylls / pharmacology
IF 4.073
リソース情報
藻類 NIES-144