RRC ID 72266
著者 Misawa N, Maoka T, Takemura M.
タイトル Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards.
ジャーナル Methods Enzymol
Abstract Authentic samples of many carotenoids are commercially available, but are often too expensive and of insufficient purity. As an alternative, a wide range of carotenoids can be biosynthesized in recombinant Escherichia coli strains. E. coli cells naturally produce the carotenoid biosynthetic precursors and provide a blank slate for manufacturing different target carotenoids, depending on the introduced genes encoding carotenoid biosynthetic enzymes. This chapter presents a series of plasmids that carry genes encoding carotenoid biosynthetic enzymes and methods to produce in E. coli 21 different carotenoids and two apocarotenoids, which can be used as authentic standards. For the structural confirmation of these carotenoids or apocarotenoids, we show their individual spectral data containing MS/MS and UV-visible, along with their biosynthetic routes in the E. coli transformants.
巻・号 670
ページ 87-137
公開日 2022-1-1
DOI 10.1016/bs.mie.2022.03.019
PII S0076-6879(22)00110-0
PMID 35871847
MeSH Carotenoids* Escherichia coli* / genetics Plasmids / genetics Tandem Mass Spectrometry
リソース情報
遺伝子材料 pAC-HIEB (RDB19495) pAC-HIEBIY (RDB19498) pAC-HIEBIYZ (RDB19499) pAC-HIEBIYZW (RDB19500) pAC-HIEBIYZG (RDB19501) pRK-HIEB (RDB19506) pRK-HIEBI (RDB19507) pRK-HIEBIY (RDB19508) pRK-HIEBIYZ (RDB19509) pRK-HIEBIYZW (RDB19510) pRK-HIEBIYZG (RDB19511) pRK-HIEBIYZ-FhCCD2 (RDB19513) pRK-HIEBIYZ-FhCCD2-NcALD8 (RDB19514)