論文 - 詳細
| RRC ID | 72276 |
|---|---|
| 著者 | Kimura Y, Ohzawa H, Miyato H, Kaneko Y, Saito A, Takahashi K, Tojo M, Yamaguchi H, Kurashina K, Saito S, Hosoya Y, Lefor AK, Sata N, Kitayama J. |
| タイトル | MiR-29b may suppresses peritoneal metastases through inhibition of the mesothelial-mesenchymal transition (MMT) of human peritoneal mesothelial cells. |
| ジャーナル | Sci Rep |
| Abstract |
Peritoneal dissemination is a major metastatic pathway for gastrointestinal and ovarian malignancies. The miR-29b family is downregulated in peritoneal fluids in patients with peritoneal metastases (PM). We examined the effect of miR-29b on mesothelial cells (MC) which play critical a role in the development of PM through mesothelial-mesenchymal transition (MMT). Human peritoneal mesothelial cells (HPMCs) were isolated from surgically resected omental tissue and MMT induced by stimulation with 10 ng/ml TGF-β1. MiR-29b mimics and negative control miR were transfected by lipofection using RNAiMAX and the effects on the MMT evaluated in vitro. HPMC produced substantial amounts of miR-29b which was markedly inhibited by TGF-β1. TGF-β1 stimulation of HPMC induced morphological changes with decreased expression of E-cadherin and calretinin, and increased expression of vimentin and fibronectin. TGF-β1 also enhanced proliferation and migration of HPMC as well as adhesion of tumor cells in a fibronectin dependent manner. However, all events were strongly abrogated by simultaneous transfection of miR-29b. MiR-29b inhibits TGF-β1 induced MMT and replacement of miR-29b in the peritoneal cavity might be effective to prevent development of PM partly through the effects on MC. |
| 巻・号 | 12(1) |
| ページ | 205 |
| 公開日 | 2022-1-7 |
| DOI | 10.1038/s41598-021-04065-2 |
| PII | 10.1038/s41598-021-04065-2 |
| PMID | 34997082 |
| PMC | PMC8742040 |
| MeSH | Animals Antigens, CD / metabolism Cadherins / metabolism Calbindin 2 / metabolism Cell Adhesion Cell Line, Tumor Cell Movement Cell Proliferation Coculture Techniques Epithelial Cells / drug effects Epithelial Cells / metabolism* Epithelial Cells / pathology Epithelial-Mesenchymal Transition* / drug effects Fibronectins / metabolism Humans Mice, Inbred C57BL MicroRNAs / genetics MicroRNAs / metabolism Peritoneal Neoplasms / genetics Peritoneal Neoplasms / metabolism* Peritoneal Neoplasms / secondary Peritoneum / drug effects Peritoneum / metabolism* Peritoneum / pathology Transforming Growth Factor beta1 / pharmacology Vimentin / metabolism |
| IF | 3.998 |
| リソース情報 | |
| ヒト・動物細胞 | NUGC-4(RCB1939) MKN45(RCB1001) |