RRC ID 72293
Author Tanaka J, Koshimura K, Sohmiya M, Murakami Y, Kato Y.
Title Involvement of tetrahydrobiopterin in trophic effect of erythropoietin on PC12 cells.
Journal Biochem Biophys Res Commun
Abstract Tetrahydrobiopterin (BH(4)) synthesis is reported to be stimulated by nerve growth factor (NGF) in PC12 cells, suggesting involvement of BH(4) in the trophic effect of NGF. We have recently reported that erythropoietin (EPO) and BH(4) enhance survival of PC12 cells. In the present study, we investigated involvement of BH(4) in the trophic effect of EPO on PC12 cells. Cellular BH(4) content was increased by EPO (10(-10) to 10(-8) M) in a dose- and time-related manner. EPO (10(-10) to 10(-8) M) increased the viable cell number of PC12 cells. In addition to EPO, BH(4) (1, 3, and 10 microM) increased the viable cell number of PC12 cells. Administration of 0.3 mM 2,4-diamino-6-hydroxypyrimidine, an inhibitor of BH(4) synthesis, blunted EPO-induced increases in BH(4) content and the viable cell number of PC12 cells. These results taken together suggest that BH(4) is involved in the trophic effects of EPO on PC12 cells.
Volume 289(2)
Pages 358-62
Published 2001-11-30
DOI 10.1006/bbrc.2001.6002
PII S0006-291X(01)96002-4
PMID 11716480
MeSH Animals Biopterin / analogs & derivatives* Biopterin / metabolism* Cell Line Cell Survival / drug effects Coloring Agents / pharmacology Culture Media, Serum-Free / pharmacology Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology Erythropoietin / metabolism* Hypoxanthines / pharmacology PC12 Cells Protein Binding Rats Tetrazolium Salts / pharmacology Thiazoles / pharmacology Time Factors
Resource
Human and Animal Cells PC-12(RCB0009)