Reference - Detail
| RRC ID | 72298 |
|---|---|
| Author | Sun W, Hattori N, Mutai H, Toyoshima Y, Kimura H, Tanaka S, Shiota K. |
| Title | PAL31, a nuclear protein required for progression to the S phase. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
PAL31 is a nuclear protein expressed by various cell types. In the present study, the expression and function of PAL31 were examined in the cytokine-regulated growth of T and B cell lines. Treatment of the cells with mitogens [ovine PRL, recombinant rat placental lactogen-I (PL-I) and human IL-3] caused a dose-dependent increase in the expression of PAL31 mRNA in the PRL-dependent cell line Nb2, and IL-3 dependent cell line BaF3. A time-course study on synchronized Nb2 cells revealed that the expression of PAL31 is specific to the late G1 and S phases. Immunocytological studies revealed that PAL31 accumulates in the nuclei at the S phase. Furthermore, the antisense oligonucleotide for PAL31 severely inhibited the proliferation of Nb2 cells by inhibiting cells progressing to the S phase. Thus, PAL31 is a nuclear protein associated with cell cycle progression. |
| Volume | 280(4) |
| Pages | 1048-54 |
| Published | 2001-2-2 |
| DOI | 10.1006/bbrc.2000.4244 |
| PII | S0006-291X(00)94244-X |
| PMID | 11162633 |
| MeSH | Animals B-Lymphocytes / metabolism* Blotting, Western Cell Cycle Cell Cycle Proteins* Cell Division Cell Line Dose-Response Relationship, Drug Flow Cytometry Fluorescent Antibody Technique G1 Phase Humans Immunohistochemistry Interleukin-3 / pharmacology Molecular Chaperones Nerve Tissue Proteins* Nuclear Proteins / biosynthesis* Nuclear Proteins / physiology* Oligonucleotides, Antisense / metabolism Placental Lactogen / pharmacology Prolactin / pharmacology RNA, Messenger / metabolism Rats S Phase* Sheep T-Lymphocytes / metabolism* Time Factors Tumor Cells, Cultured |
| IF | 2.985 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Ba/F3(RCB0805) WEHI -3(RCB0035) |