| RRC ID |
72300
|
| Author |
Hasegawa T, Feijoo Carnero C, Wada T, Itoyama Y, Miyagi T.
|
| Title |
Differential expression of three sialidase genes in rat development.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Mammalian sialidases have been reported to give a great influence on a number of cellular functions including cell differentiation and cell growth by removal of sialic acids from glycoproteins and gangliosides. To understand the roles of the sialidases during development, we investigated expression pattern of three types of sialidase in developing rat brain and liver. For this purpose we cloned a new membrane-associated sialidase cDNA from rat brain. The cDNA encodes 418 amino acids containing three ASP-boxes characteristic of sialidases and the major transcript of 3.5 kb is highly expressed in brain and cardiac muscle but low in liver. Competitive polymerase chain reaction methods were developed to evaluate the mRNA level together with activity assays in comparison with cytosolic and lysosomal sialidases previously obtained. The results indicate that the expression of individual sialidase genes is spatiotemporally controlled with distinct roles in determining the concentration and components of sialo-glycoconjugates during development.
|
| Volume |
280(3)
|
| Pages |
726-32
|
| Published |
2001-1-26
|
| DOI |
10.1006/bbrc.2000.4186
|
| PII |
S0006291X0094186X
|
| PMID |
11162581
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Brain / embryology
Brain / enzymology
Brain / growth & development
DNA Primers / genetics
DNA, Complementary / genetics
Gene Expression Regulation, Developmental
Gene Expression Regulation, Enzymologic
Liver / embryology
Liver / enzymology
Liver / growth & development
Membranes / enzymology
Molecular Sequence Data
Neuraminidase / genetics*
Neuraminidase / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
Rats
Rats, Sprague-Dawley
|
| IF |
2.985
|
| Resource |
| Human and Animal Cells |
COS-1(RCB0143) |
