論文 - 詳細
| RRC ID | 72319 |
|---|---|
| 著者 | Fukui K, Arai K, Tanaka Y, Aoi Y, Kukshal V, Jez JM, Kubes MF, Napier R, Zhao Y, Kasahara H, Hayashi KI. |
| タイトル | Chemical inhibition of the auxin inactivation pathway uncovers the roles of metabolic turnover in auxin homeostasis. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
The phytohormone auxin, indole-3-acetic acid (IAA), plays a prominent role in plant development. Auxin homeostasis is coordinately regulated by auxin synthesis, transport, and inactivation; however, the physiological contribution of auxin inactivation to auxin homeostasis has not been determined. The GH3 IAA-amino acid conjugating enzymes play a central role in auxin inactivation. Chemical inhibition of GH3 proteins in planta is challenging because the inhibition of these enzymes leads to IAA overaccumulation that rapidly induces GH3 expression. Here, we report the characterization of a potent GH3 inhibitor, kakeimide, that selectively targets IAA-conjugating GH3 proteins. Chemical knockdown of the auxin inactivation pathway demonstrates that auxin turnover is very rapid (about 10 min) and indicates that both auxin biosynthesis and inactivation dynamically regulate auxin homeostasis. |
| 巻・号 | 119(32) |
| ページ | e2206869119 |
| 公開日 | 2022-8-9 |
| DOI | 10.1073/pnas.2206869119 |
| PMID | 35914172 |
| PMC | PMC9371723 |
| MeSH | Arabidopsis Homeostasis* Indoleacetic Acids* / metabolism Plant Growth Regulators / metabolism |
| IF | 9.412 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda08963 pda05782 pda02630 pda10271 pda03475 |