| RRC ID |
72325
|
| Author |
Nakazato T, Kawamura T, Uemura T, Liu L, Li J, Sasai M, Harada A, Ito E, Iseoka H, Toda K, Sawa Y, Miyagawa S.
|
| Title |
Engineered three-dimensional cardiac tissues maturing in a rotating wall vessel bioreactor remodel diseased hearts in rats with myocardial infarction.
|
| Journal |
Stem Cell Reports
|
| Abstract |
A rotating wall vessel (RWV) bioreactor was constructed for growing massive functional cardiac constructs to recover the function of a distressed rat heart. Three-dimensional cardiac tissues were engineered by seeding human-induced pluripotent stem cell-derived cardiomyocytes on poly(lactic-co-glycolic acid) fiber sheets (3D-hiPSC-CTs) and cultured in the RWV bioreactor (RWV group) or under static conditions (control group). The tissues were transplanted into a myocardial infarction nude rat model, and cardiac performance was evaluated. In the RWV group, cell viability and contractile and electrical properties significantly improved, mature cardiomyocytes were observed, and mechanical stress-related mediators of mammalian target of rapamycin signaling were upregulated compared with those of the control. Four weeks post-transplantation, tissue survival and left ventricular ejection fraction significantly improved in the RWV group. Hence, dynamic culture in an RWV bioreactor could provide a superior culture environment for improved performance of 3D-hiPSC-CTs, providing a means for functional cardiomyogenesis in myocyte-loss heart failure.
|
| Volume |
17(5)
|
| Pages |
1170-1182
|
| Published |
2022-5-10
|
| DOI |
10.1016/j.stemcr.2022.03.012
|
| PII |
S2213-6711(22)00145-X
|
| PMID |
35427484
|
| PMC |
PMC9133656
|
| MeSH |
Animals
Bioreactors
Mammals
Myocardial Infarction* / therapy
Myocytes, Cardiac / transplantation
Rats
Rats, Nude
Stroke Volume
Tissue Engineering / methods
Ventricular Function, Left*
|
| IF |
6.032
|
| Resource |
| Human and Animal Cells |
253G1(HPS0002) |
