Reference - Detail
| RRC ID | 72352 |
|---|---|
| Author | Noguchi T, Ikeda K, Yamamoto K, Yoshida I, Ashiba A, Tsuchiyama J, Shinagawa K, Yoshino T, Takata M, Harada M. |
| Title | Antisense oligodeoxynucleotides to latent membrane protein 1 induce growth inhibition, apoptosis and Bcl-2 suppression in Epstein-Barr virus (EBV)-transformed B-lymphoblastoid cells, but not in EBV-positive natural killer cell lymphoma cells. |
| Journal | Br J Haematol |
| Abstract |
Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP-1) is essential for immortalization of B cells by EBV, protects the infected cells from apoptotic cell death and induces Bcl-2 expression. Suppression of LMP-1 expression by antisense oligodeoxynucleotides (AS-oligo) to LMP-1 inhibits proliferation, promotes apoptosis and suppresses Bcl-2 expression in EBV-transformed B cells. However, the function of LMP-1 expression in EBV-positive natural killer (NK) cell lymphoma cells has not been reported previously. We examined the function of LMP-1 in two EBV-positive NK cell lymphoma cell lines (NK-YS and YT) through suppressing LMP-1 expression by AS-oligo to LMP-1. The AS-oligo to LMP-1 suppressed LMP-1 mRNA and protein expression in two EBV-positive NK cell lymphoma cell lines, as well as in an EBV-transformed B-cell line (CMG-1). Proliferation was inhibited, apoptosis was induced and Bcl-2 expression was suppressed in CMG-1 cells, but none of these events were observed in NK-YS or YT cells. These results suggest that proliferation, inhibition of apoptosis and Bcl-2 expression in EBV-positive NK cell lymphoma cells are not directly regulated by LMP-1 as in EBV-transformed B-cell lines, but are probably mediated through other signal transducing systems. |
| Volume | 114(1) |
| Pages | 84-92 |
| Published | 2001-7-1 |
| DOI | 10.1046/j.1365-2141.2001.02887.x |
| PII | bjh2887 |
| PMID | 11472349 |
| MeSH | Adaptor Proteins, Signal Transducing Apoptosis / drug effects Blotting, Western Carrier Proteins / genetics* Cell Division / drug effects Cell Line, Transformed Cytoskeletal Proteins Gene Deletion Gene Expression / drug effects Herpesvirus 4, Human* Humans Intracellular Signaling Peptides and Proteins Killer Cells, Natural / metabolism Killer Cells, Natural / virology* LIM Domain Proteins Lymphoma, T-Cell / metabolism Lymphoma, T-Cell / virology* Oligonucleotides, Antisense / pharmacology* Proto-Oncogene Proteins c-bcl-2 / genetics Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured |
| IF | 5.518 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | CGM1(RCB0566) |