| RRC ID |
72354
|
| Author |
Sakamoto M, Ota K, Kondo Y, Okamura M, Fujii H, Bito H.
|
| Title |
In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice.
|
| Journal |
STAR Protoc
|
| Abstract |
We present a protocol to prepare mouse cranial window implantation for in vivo two-photon wide-field calcium imaging. This protocol uses G-CaMP9a transgenic mice, which express a genetically encoded calcium indicator with high signal-to-noise ratio. We describe in utero electroporation, followed by headplate fixation and cranial window implantation. This protocol can be used for measuring neural activity and is suitable for long-term imaging in large populations. Moreover, this protocol does not require preparation of Flp-expressing transgenic mice. For complete details on the use and execution of this protocol, please refer to Sakamoto et al. (2022).
|
| Volume |
3(2)
|
| Pages |
101421
|
| Published |
2022-6-17
|
| DOI |
10.1016/j.xpro.2022.101421
|
| PII |
S2666-1667(22)00301-X
|
| PMID |
35693213
|
| PMC |
PMC9185017
|
| MeSH |
Animals
Calcium, Dietary
Diagnostic Imaging
Electroporation*
Mice
Mice, Transgenic
Skull* / surgery
|
| Resource |
| DNA material |
pCAG-Flpo (RDB19603)
pCAG-mCherry (RDB19604) |
