論文 - 詳細
| RRC ID | 72363 |
|---|---|
| 著者 | Kimura Y, Okuda H. |
| タイトル | Resveratrol isolated from Polygonum cuspidatum root prevents tumor growth and metastasis to lung and tumor-induced neovascularization in Lewis lung carcinoma-bearing mice. |
| ジャーナル | J Nutr |
| Abstract |
Resveratrol is a naturally occurring phytoalexine found in medicinal plants. We found that resveratrol, at doses of 2.5 and 10 mg/kg, significantly reduced the tumor volume (42%), tumor weight (44%) and metastasis to the lung (56%) in mice bearing highly metastatic Lewis lung carcinoma (LLC) tumors, but not at a dose of 0.6 mg/kg. Resveratrol did not affect the number of CD4(+), CD8(+) and natural killer (NK)1.1.(+) T cells in the spleen. Therefore, the inhibitory effects of resveratrol on tumor growth and lung metastasis could not be explained by natural killer or cytotoxic T-lymphocyte activation. In addition, resveratrol inhibited DNA synthesis most strongly in LLC cells; its 50% inhibitory concentration (IC(50)) was 6.8 micromol/L. Resveratrol at 100 micromol/L increased apoptosis to 20.6 +/- 1.35% from 12.1 +/- 0.36% (P < 0.05) in LLC cells, and decreased the S phase population to 22.1 +/- 1.03% and 29.2 +/- 0.27% from 35.2 +/- 1.72% (P < 0.05) at concentrations of 50 and 100 micromol/L, respectively. Resveratrol inhibited tumor-induced neovascularization at doses of 2.5 and 10 mg/kg in an in vivo model. Moreover, resveratrol significantly inhibited the formation of capillary-like tube formation from human umbilical vein endothelial cells (HUVEC) at concentrations of 10-100 micromol/L; the degree of the inhibition of capillary-like tube formation by resveratrol was 45.5% at 10 micromol/L, 50.2% at 50 micromol/L and 52.6% at 100 micromol/L. Resveratrol inhibited the binding of vascular endothelial growth factor (VEGF) to HUVEC at concentrations of 10-100 micromol/L, but not at concentrations of 1 and 5 micromol/L. The degree of inhibition of VEGF binding to HUVEC by resveratrol was 16.9% at 10 micromol/L, 53.2% at 50 micromol/L and 47.8% at 100 micromol/L. We suggest that the antitumor and antimetastatic activities of resveratrol might be due to the inhibition of DNA synthesis in LLC cells and the inhibition of LLC-induced neovascularization and tube formation (angiogensis) of HUVEC by resveratrol |
| 巻・号 | 131(6) |
| ページ | 1844-9 |
| 公開日 | 2001-6-1 |
| DOI | 10.1093/jn/131.6.1844 |
| PMID | 11385077 |
| MeSH | Angiogenesis Inhibitors / pharmacology* Animals Anticarcinogenic Agents / pharmacology* Apoptosis Body Weight CD4-CD8 Ratio Carcinoma, Lewis Lung / drug therapy* Carcinoma, Lewis Lung / pathology Cell Cycle DNA / biosynthesis Disease Models, Animal Female Lung Neoplasms / drug therapy* Lung Neoplasms / prevention & control Lung Neoplasms / secondary Mice Mice, Inbred C57BL Neoplasm Metastasis Neovascularization, Pathologic / prevention & control Organ Size Polygonaceae / chemistry* Resveratrol Spleen / immunology Spleen / pathology Stilbenes / isolation & purification Stilbenes / pharmacology* Thymus Gland / pathology Tumor Cells, Cultured |
| IF | 4.281 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | LLC(RCB0558) |