| RRC ID |
72384
|
| 著者 |
Yano K, Shibata O, Mizuno A, Kobayashi F, Higashio K, Morinaga T, Tsuda E.
|
| タイトル |
Immunological study on circulating murine osteoprotegerin/osteoclastogenesis inhibitory factor (OPG/OCIF): possible role of OPG/OCIF in the prevention of osteoporosis in pregnancy.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Osteoprotegerin (OPG)/osteoclastogenesis inhibitory factor (OCIF) is a soluble member of the tumor necrosis factor receptor family and plays a crucial role in the negative regulation of osteoclastic bone resorption. We have immunized OPG/OCIF knockout mice with murine rOPG/rOCIF and established a panel of hybridomas producing monoclonal antibodies (mAbs) to murine rOPG/rOCIF. Utilizing the mAbs, we developed enzyme-linked immunosorbent assay (ELISA) systems: one detecting both homodimeric and monomeric forms of murine OPG/OCIF and the other detecting only dimeric form of murine OPG/OCIF. With the aid of these ELISA systems we showed that OPG/OCIF is present mainly as a monomer in murine blood. The concentration of OPG/OCIF in normal mouse sera was approximately 500 pg/ml and there was no statistical difference in the serum concentration of OPG/OCIF among genders, age, and strains. Interestingly, the concentration of circulating OPG/OCIF in mouse markedly increased during pregnancy. The result indicated that circulating OPG/OCIF plays an important role in the protection of bone from excess resorption during pregnancy in mammals.
|
| 巻・号 |
288(1)
|
| ページ |
217-24
|
| 公開日 |
2001-10-19
|
| DOI |
10.1006/bbrc.2001.5745
|
| PII |
S0006-291X(01)95745-6
|
| PMID |
11594776
|
| MeSH |
Animals
Antibodies, Monoclonal / immunology
Biomarkers / blood
CHO Cells
Cricetinae
Dimerization
Enzyme-Linked Immunosorbent Assay / methods*
Female
Glycoproteins / blood*
Glycoproteins / immunology
Glycoproteins / physiology*
Mice
Mice, Knockout
Osteoporosis / prevention & control*
Osteoprotegerin
Pregnancy
Pregnancy, Animal / blood*
Receptors, Cytoplasmic and Nuclear / blood*
Receptors, Cytoplasmic and Nuclear / immunology
Receptors, Cytoplasmic and Nuclear / physiology*
Receptors, Tumor Necrosis Factor
|
| IF |
2.985
|
| リソース情報 |
| ヒト・動物細胞 |
ST2(RCB0224) |
