論文 - 詳細
| RRC ID | 72384 |
|---|---|
| 著者 | Yano K, Shibata O, Mizuno A, Kobayashi F, Higashio K, Morinaga T, Tsuda E. |
| タイトル | Immunological study on circulating murine osteoprotegerin/osteoclastogenesis inhibitory factor (OPG/OCIF): possible role of OPG/OCIF in the prevention of osteoporosis in pregnancy. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Osteoprotegerin (OPG)/osteoclastogenesis inhibitory factor (OCIF) is a soluble member of the tumor necrosis factor receptor family and plays a crucial role in the negative regulation of osteoclastic bone resorption. We have immunized OPG/OCIF knockout mice with murine rOPG/rOCIF and established a panel of hybridomas producing monoclonal antibodies (mAbs) to murine rOPG/rOCIF. Utilizing the mAbs, we developed enzyme-linked immunosorbent assay (ELISA) systems: one detecting both homodimeric and monomeric forms of murine OPG/OCIF and the other detecting only dimeric form of murine OPG/OCIF. With the aid of these ELISA systems we showed that OPG/OCIF is present mainly as a monomer in murine blood. The concentration of OPG/OCIF in normal mouse sera was approximately 500 pg/ml and there was no statistical difference in the serum concentration of OPG/OCIF among genders, age, and strains. Interestingly, the concentration of circulating OPG/OCIF in mouse markedly increased during pregnancy. The result indicated that circulating OPG/OCIF plays an important role in the protection of bone from excess resorption during pregnancy in mammals. |
| 巻・号 | 288(1) |
| ページ | 217-24 |
| 公開日 | 2001-10-19 |
| DOI | 10.1006/bbrc.2001.5745 |
| PII | S0006-291X(01)95745-6 |
| PMID | 11594776 |
| MeSH | Animals Antibodies, Monoclonal / immunology Biomarkers / blood CHO Cells Cricetinae Dimerization Enzyme-Linked Immunosorbent Assay / methods* Female Glycoproteins / blood* Glycoproteins / immunology Glycoproteins / physiology* Mice Mice, Knockout Osteoporosis / prevention & control* Osteoprotegerin Pregnancy Pregnancy, Animal / blood* Receptors, Cytoplasmic and Nuclear / blood* Receptors, Cytoplasmic and Nuclear / immunology Receptors, Cytoplasmic and Nuclear / physiology* Receptors, Tumor Necrosis Factor |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) |