RRC ID 72385
Author Yonemitsu J.
Title Interleukin-4 induces mouse cytomegalovirus interstitial pneumonia in a latent infection model.
Journal Kurume Med J
Abstract To better understand immune mechanisms involved in onset of cytomegalovirus pneumonia, we initially examined the replication of a low virulence strain of mouse cytomegalovirus (MCMV) in nude and BALB/c mice infected by intranasal inoculation. MCMV was detected by plaque assay in the salivary glands of nude mice from days 3 to 16, and in those of BALB/c mice from days 7 to 11. Nude mice became infected with MCMV earlier than BALB/c mice. Moreover, MCMV-DNA was detected in the salivary glands until day 16 after MCMV inoculation in nude and BALB/c mice. However, we did not find evidence of interstitial pneumonia at day 16 in either BALB/c or nude mice. These results suggest that this system represents a latent infection model in BALB/c mice and a persistent infection model in nude mice. We treated latently infected BALB/c mice with methylprednisolone or IL-4 every other day. The mice treated with IL-4 developed interstitial pneumonia, whereas those treated with m-PSL did not. In the present study, we constructed a model of MCMV latent infection that could be used to induce development of interstitial pneumonia. IL-4 appears to be a key cytokine for onset of interstitial pneumonia in mice with latent MCMV infection.
Volume 48(1)
Pages 49-54
Published 2001-1-1
DOI 10.2739/kurumemedj.48.49
PMID 11402620
MeSH Animals DNA, Viral / analysis Disease Models, Animal Herpesviridae Infections / etiology* Herpesviridae Infections / immunology Herpesviridae Infections / virology Interleukin-4 / pharmacology* Lung Diseases, Interstitial / etiology* Lung Diseases, Interstitial / immunology Lung Diseases, Interstitial / virology Male Methylprednisolone / pharmacology Mice Mice, Inbred BALB C Muromegalovirus / physiology* Virus Replication
Human and Animal Cells C127(RCB0036)