RRC ID 72423
著者 Hotta H, Hamamura K, Shibuya H, Ohmi Y, Furukawa K, Furukawa K.
タイトル Lewis y Expressed in Oral Squamous Cell Carcinoma Attenuates Malignant Properties via Down-regulation of EGF Signaling.
ジャーナル Anticancer Res
Abstract BACKGROUND/AIM:Lewis y is expressed in oral squamous cell carcinoma (OSCC) cells and tumors. Previously, we reported that Lewis y was not expressed in invasion areas, and attenuation of proliferation and invasion in OSCC cells was caused by over-expression of Lewis y. However, the roles of Lewis y in the attenuation of malignant properties have not been clarified. In this study, we investigated the roles of Lewis y in OSCC.
MATERIALS AND METHODS:The levels of Lewis y on EGFR and the phosphorylation levels of EGFR in OSCC cells were analyzed by immunoprecipitation and western blot. EGFR cross-linking and binding kinetics of EGF were performed.
RESULTS:Upon EGF stimulation, phosphorylation and dimer formation of EGFR were more prominent in Lewis y- cells. EGF binding kinetics showed reduced binding sites in Lewis y+ cells.
CONCLUSION:Lewis y reduced EGF binding to EGFR, leading to suppression of malignant properties through suppression of EGF signaling.
巻・号 41(4)
ページ 1821-1830
公開日 2021-4-1
DOI 10.21873/anticanres.14948
PII 41/4/1821
PMID 33813387
MeSH Binding Sites Cell Adhesion Cell Line, Tumor Cell Movement Cell Shape Epidermal Growth Factor / metabolism* ErbB Receptors / metabolism Humans Kinetics Lewis Blood Group Antigens / metabolism* Mouth Neoplasms / metabolism* Mouth Neoplasms / pathology Neoplasm Invasiveness Phosphorylation Protein Binding Signal Transduction Squamous Cell Carcinoma of Head and Neck / metabolism* Squamous Cell Carcinoma of Head and Neck / pathology
IF 1.994
リソース情報
ヒト・動物細胞 HSC-2(RCB1945) HSC-3(RCB1975) HSC-4(RCB1902) Sa3(RCB0980) SCCKN(RCB0441) HO-1-u-1(RCB2102) Ca9-22(RCB1976)