Reference - Detail
RRC ID | 72423 |
---|---|
Author | Hotta H, Hamamura K, Shibuya H, Ohmi Y, Furukawa K, Furukawa K. |
Title | Lewis y Expressed in Oral Squamous Cell Carcinoma Attenuates Malignant Properties via Down-regulation of EGF Signaling. |
Journal | Anticancer Res |
Abstract |
BACKGROUND/AIM:Lewis y is expressed in oral squamous cell carcinoma (OSCC) cells and tumors. Previously, we reported that Lewis y was not expressed in invasion areas, and attenuation of proliferation and invasion in OSCC cells was caused by over-expression of Lewis y. However, the roles of Lewis y in the attenuation of malignant properties have not been clarified. In this study, we investigated the roles of Lewis y in OSCC. MATERIALS AND METHODS:The levels of Lewis y on EGFR and the phosphorylation levels of EGFR in OSCC cells were analyzed by immunoprecipitation and western blot. EGFR cross-linking and binding kinetics of EGF were performed. RESULTS:Upon EGF stimulation, phosphorylation and dimer formation of EGFR were more prominent in Lewis y- cells. EGF binding kinetics showed reduced binding sites in Lewis y+ cells. CONCLUSION:Lewis y reduced EGF binding to EGFR, leading to suppression of malignant properties through suppression of EGF signaling. |
Volume | 41(4) |
Pages | 1821-1830 |
Published | 2021-4-1 |
DOI | 10.21873/anticanres.14948 |
PII | 41/4/1821 |
PMID | 33813387 |
MeSH | Binding Sites Cell Adhesion Cell Line, Tumor Cell Movement Cell Shape Epidermal Growth Factor / metabolism* ErbB Receptors / metabolism Humans Kinetics Lewis Blood Group Antigens / metabolism* Mouth Neoplasms / metabolism* Mouth Neoplasms / pathology Neoplasm Invasiveness Phosphorylation Protein Binding Signal Transduction Squamous Cell Carcinoma of Head and Neck / metabolism* Squamous Cell Carcinoma of Head and Neck / pathology |
IF | 1.994 |
Resource | |
Human and Animal Cells | HSC-2(RCB1945) HSC-3(RCB1975) HSC-4(RCB1902) Sa3(RCB0980) SCCKN(RCB0441) HO-1-u-1(RCB2102) Ca9-22(RCB1976) |