RRC ID 72466
Author Okamoto H, Takano M, Yokoyama K, Kondo J, Yayama K.
Title Fibroblasts synthesize kininogen in response to inflammatory mediators.
Journal Immunopharmacology
Abstract Mouse fibroblasts in vitro secret kininogen (KGN). Rat fibroblasts also synthesized and secreted T-KGN in vitro. KGN production by these fibroblasts is greatly stimulated by dibutyryl cAMP, prostaglandin E2 and tumor necrosis factor. Human fibroblast WI-38 cells also express the L-KGN gene, which was stimulated by dibutyryl cAMP and prostaglandin E2. These results demonstrate that fibroblasts express the KGN gene, and suggest that the expression is regulated by inflammatory mediators. RT-PCR, using specific primers for the T-KGN and L-KGN genes, reveals that the rat hind-paw express both T- and L-KGN mRNAs, and the expression of both KGN mRNAs was increased in the hind-paw 24 h after inducing inflammation by injecting Freund's complete adjuvant into the paw. Thus, it is suggested that local connective tissues express the KGN gene, and that the expression is enhanced under pathological conditions, such as inflammation.
Volume 32(1-3)
Pages 28-33
Published 1996-5-1
DOI 10.1016/0162-3109(96)00005-7
PII 0162-3109(96)00005-7
PMID 8796262
MeSH Animals Cell Line Fibroblasts / drug effects* Fibroblasts / metabolism* Humans Inflammation Mediators / pharmacology* Kininogens / biosynthesis* Kininogens / genetics Mice Mice, Inbred ICR Rats Rats, Sprague-Dawley
Human and Animal Cells KGN(RCB1154)