| RRC ID |
72513
|
| Author |
Kondo Y, Yoshihashi S, Mimori K, Ogihara R, Kanehama Y, Maki Y, Enosawa S, Kurose K, Iwao T, Nakamura K, Matsunaga T.
|
| Title |
Selective culture method for hepatocyte-like cells differentiated from human induced pluripotent stem cells.
|
| Journal |
Drug Metab Pharmacokinet
|
| Abstract |
This study aimed to establish culture conditions which are able to give the differentiation of induced pluripotent (iPS) cells to hepatocytes. To this end, we examined the usefulness of a culture medium containing the components involved in the intermediary metabolism in the liver. More specifically, we examined the effect of the "modified L-15 medium" containing galactose, phenylalanine and ornitine, but deprived of glucose, tyrosine, arginine and pyruvic acid. The medium was altered according to changes in the expression of enzymes that participate in liver-specific pathways. After 25 days of differentiation, the differentiated cells expressed hepatocyte markers and drug-metabolizing enzymes. These expression levels were increased using modified L-15 medium. The survival of human fetal liver cells and the death of human fibroblasts were observed during culture in modified L-15 medium. Most of the cells that differentiated from human iPS cells using modified L-15 medium were stained by anti-human albumin antibody. These results suggest that iPS cells can be converted to high purity-differentiated hepatocytes by cultivating them in modified L-15 medium.
|
| Volume |
29(5)
|
| Pages |
407-13
|
| Published |
2014-1-1
|
| DOI |
10.2133/dmpk.dmpk-14-rg-022
|
| PII |
DN/JST.JSTAGE/dmpk/DMPK-14-RG-022
|
| PMID |
24785642
|
| MeSH |
Cell Culture Techniques*
Cell Differentiation* / drug effects
Cell Survival / drug effects
Culture Media / chemistry
Culture Media / pharmacology
Hepatocytes / cytology*
Hepatocytes / drug effects
Hepatocytes / enzymology
Hepatocytes / metabolism
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / drug effects
|
| IF |
2.772
|
| Resource |
| Human and Animal Cells |
Hep G2(RCB1886) |
