論文 - 詳細
| RRC ID | 72514 |
|---|---|
| 著者 | Hirai H, Shogaki Y, Sato M. |
| タイトル | The Mis6 inner kinetochore subcomplex maintains CENP-A nucleosomes against centromeric non-coding transcription during mitosis. |
| ジャーナル | Commun Biol |
| Abstract |
Centromeres are established by nucleosomes containing the histone H3 variant CENP-A. CENP-A is recruited to centromeres by the Mis18-HJURP machinery. During mitosis, CENP-A recruitment ceases, implying the necessity of CENP-A maintenance at centromeres, although the exact underlying mechanism remains elusive. Herein, we show that the inner kinetochore protein Mis6 (CENP-I) and Mis15 (CENP-N) retain CENP-A during mitosis in fission yeast. Eliminating Mis6 or Mis15 during mitosis caused immediate loss of pre-existing CENP-A at centromeres. CENP-A loss occurred due to the transcriptional upregulation of non-coding RNAs at the central core region of centromeres, as confirmed by the observation RNA polymerase II inhibition preventing CENP-A loss from centromeres in the mis6 mutant. Thus, we concluded that the inner kinetochore complex containing Mis6-Mis15 blocks the indiscriminate transcription of non-coding RNAs at the core centromere, thereby retaining the epigenetic inheritance of CENP-A during mitosis. |
| 巻・号 | 5(1) |
| ページ | 818 |
| 公開日 | 2022-8-15 |
| DOI | 10.1038/s42003-022-03786-y |
| PII | 10.1038/s42003-022-03786-y |
| PMID | 35970865 |
| PMC | PMC9378642 |
| MeSH | Carrier Proteins / metabolism Cell Cycle Proteins / metabolism Centromere / metabolism Centromere Protein A / genetics Chromosomal Proteins, Non-Histone / genetics Chromosomal Proteins, Non-Histone / metabolism Kinetochores / metabolism Mitosis Nucleosomes / genetics Nucleosomes / metabolism Schizosaccharomyces* / genetics Schizosaccharomyces* / metabolism Schizosaccharomyces pombe Proteins* / genetics |
| IF | 4.165 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY336 |